Recombinant PKAr-1a (from E. coli)

: Biorbyt

Recombinant PKAr-1a (from E. coli)

: Biorbyt

Protein/peptide type:
Recombinant
Source:
E. coli
Storage conditions:
PKA should be stored at 4 °C if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at −20 °C.Avoid multiple freeze-thaw cycles.
Biological activity:
PKA regulatory subunit alpha specifically inhibits PKA catalytic subunit (Ki about 0.7nM). The binding is not dependent on the presence of ATPMg, however, the regulatory subunit will be phosphorylated at the binding site by PKA alpha upon binding. Activity can be restored by adding the second messenger cAMP (Kact about 100nM). Subcellular localisation is regulated via interaction of PKA RII with so-called A-kinase anchoring proteins (AKAPs).
Protein synonyms:
cAMP-Dependent
Protein/peptide name:
PKAr-1a
Purity:
Greater than 95% as determined by SDS-PAGE.
Formulation:
PKA regulatory subunit-II alpha is supplied in 50% glycerol.
Tested applications:
SDS-PAGE

orb80851

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