Unconjugated

Recombinant

E. coli

Human

Store at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.

ED50 is less than 0.03 ng/ml as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
Specific Activity of 3.0 x 107 IU/mg.

APC1

P01375 (Human)

TNF alpha

>95% as determined by reducing SDS-PAGE.

MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLF KGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKG DRLSAEINRPDYLDFAESGQVYFGIIAL

Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.0.

SDS-PAGE , FuncS