Human TNF alpha, Mutant (from E. coli)
: Biorbyt
- Source:E. coli
- Species:Human
- Storage conditions:Store at 4 °C for up to two weeks. For long term storage, aliquot and store at −20 °C, avoid freeze/thaw cycles.
- Biological activity:The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin is 0.05ng/ml, corresponding to Specific Activity of 100,000,000 IU/mg.
- Protein synonyms:APC1
- Protein/peptide name:TNF alpha, Mutant
- Purity:> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
- Solubility:It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha Variant in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
- Sequence:MRKRKPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDNQLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIAF.
- Formulation:The protein was lyophilized after extensive dialysis against 0.5x PBS pH -7.
- Tested applications:SDS-PAGE , HPLC , FuncS
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