PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.

• Highly reproducible, colorimetric assay
• Linear range: 0 to 50 µM
• Enzyme independent
• Measure lipid peroxidation and monitor protein glycation

PEROXsay™ is suitable for quantifying the level of protein-damaging peroxides in biological solutions, measurement of lipid peroxidation of low density lipoproteins and liposomes and monitoring protein glycation. Designed for microtitre plates, but can be scaled up for use with 1 ml cuvettes.

PEROXsay™-LIPID permits the quantification of peroxides in the presence of lipids without the need for extraction steps.