Affinity chromatography columns, HisTrap™ FF crude
About this item
HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
- High binding capacity – binds up to 40 mg his-tag protein/mL resin
- Excellent flow properties – great performance even at high flow rates
- Optimized for unclarified samples – short purification time can minimize potential negative effects, such as degradation and oxidation of proteins
- HiTrap column format – compatible with syringe, pump, or chromatography system such as ÄKTA systems or other FPLC systems
HisTrap FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. After thorough cell disruption, it is possible to load the unclarified lysate on the column, without precentrifugation and filtration.
3 Options Available Below
Product Details & Documents
HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
- High binding capacity – binds up to 40 mg his-tag protein/mL resin
- Excellent flow properties – great performance even at high flow rates
- Optimized for unclarified samples – short purification time can minimize potential negative effects, such as degradation and oxidation of proteins
- HiTrap column format – compatible with syringe, pump, or chromatography system such as ÄKTA systems or other FPLC systems
HisTrap FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. After thorough cell disruption, it is possible to load the unclarified lysate on the column, without precentrifugation and filtration.
Specifications for Product Family
Specifications
- Catalog No:
- 11-0004-58
- 29-0486-31
- 17-5286-01
- Binding capacity:
- Approx. 40 mg histidine-tagged protein/mL medium
- Approx. 40 mg histidine-tagged protein/mL medium
- Approx. 40 mg histidine-tagged protein/mL medium
- Max. pressure:
- 5 bar
- 5 bar
- 5 bar
- pH range:
- 3 - 12
- 3 - 12
- 3 - 12
- Storage:
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
Specifications
Product Family Options
Product Information
- Column hardwareParticle SizeBed dimensionsCapacityFlow rateAvailabilityPrice
- 11-0004-58HisTrap™ FF crudeSpecifications:
More
Specifications
Cat. No.11-0004-58Binding capacityApprox. 40 mg histidine-tagged protein/mL mediumMax. pressure5 barpH range3 - 12Storage4 to 30 °C, 20% Ethanol - 29-0486-31HisTrap™ FF crudeSpecifications:
More
Specifications
Cat. No.29-0486-31Binding capacityApprox. 40 mg histidine-tagged protein/mL mediumMax. pressure5 barpH range3 - 12Storage4 to 30 °C, 20% Ethanol - 17-5286-01HisTrap™ FF crudeSpecifications:
More
Specifications
Cat. No.17-5286-01Binding capacityApprox. 40 mg histidine-tagged protein/mL mediumMax. pressure5 barpH range3 - 12Storage4 to 30 °C, 20% Ethanol
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