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184 results for Electrophoresis Reagents

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Electrophoresis Reagents

Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

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RNA ladder, high range, RiboRuler™

RNA ladder, high range, RiboRuler™

Supplier: Thermo Fisher Scientific

RiboRuler™ high range RNA ladder allows precise sizing of RNA molecules in denaturing agarose gels and Northern blotting.

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Destain solutions

Destain solutions

Supplier: G-Biosciences

G-Biosciences' Destain I contains approximately 45% methanol and 9% glacial acetic acid. G-Biosciences' Destain II contains approximately 5% methanol and 7% acetic acid.

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Far-western blot kit for biotinylated proteins, Pierce™

Far-western blot kit for biotinylated proteins, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Far-Western Blot Kit for Biotinylated Proteins enables in-gel or on-membrane Western blot detection of target proteins through interaction with any biotin-labeled test protein used as the primary probe. On-membrane detection offers greater sensitivity whereas in-gel detection is faster and prevents problems associated with incomplete or inefficient transfer.

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Fedezze fel az Alfa Aesa és Arcor termékeit nálunk!

Vásárolja meg Acros & Alfa Aesar vegyszereit az Avantor®-tól, hogy optimalizálja laboratóriuma ellátását

Már bízik a Thermo Fisher kiváló minőségű vegyi anyagaiban, valamint az Avantor® kiváló szolgáltatásában és támogatásában. Nos, miért ne kombinálná a kettőt?

Vásároljon most!

Pierce™ Biotin 3' End DNA Labelling Kit

Pierce™ Biotin 3' End DNA Labelling Kit

Supplier: Thermo Fisher Scientific

This Biotin 3' End DNA Labelling Kit is used for tagging single-stranded DNA primers with biotin for use in non radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.

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Pierce™ Western Blot Signal Enhancer

Pierce™ Western Blot Signal Enhancer

Supplier: Thermo Fisher Scientific

Western blot signal enhancer is a membrane treatment that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a Level at which it could not be detected without enhancement.

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Fluorogenic peroxidase substrate kit, QuantaBlu™

Fluorogenic peroxidase substrate kit, QuantaBlu™

Supplier: Thermo Fisher Scientific

QuantaBlu™ Fluorogenic peroxidase substrate kit is a three-component kit containing proprietary fluorescent substrate, peroxide and stop solutions for stopped (endpoint) or non-stopped assays.

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1-Step™, Pierce™ Chloronaphthol

1-Step™, Pierce™ Chloronaphthol

Supplier: Thermo Fisher Scientific

Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.

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1-Step™ Ultra TMB-Blotting solution, Pierce™

1-Step™ Ultra TMB-Blotting solution, Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.

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Ethidium bromide Greenbag™ disposal kit, MP Biomedicals

Supplier: MP Biomedicals

The EtBr Greenbag™ Disposal Kit allows rapid and trouble-free concentration of ethdium bromide from large volumes of solutions into a small 'teabag' containing activated carbon. The Greenbag™ is then conveniently disposed of along with other solid hazardous wastes.

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Amplite® West ECL HRP Substrate *Femto Sensitivity*

Amplite® West ECL HRP Substrate *Femto Sensitivity*

Supplier: AAT BIOQUEST

Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.

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Protein immunodetection reagents, SuperSignal™ West Dura extended duration substrate

Protein immunodetection reagents, SuperSignal™ West Dura extended duration substrate

Supplier: Thermo Fisher Scientific

The SuperSignal™ West Dura Extended Duration Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Dura Substrate enables mid-femtogram detection of antigen by oxidising luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualised on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions.

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Chemiluminescent EMSA kit, LightShift™

Chemiluminescent EMSA kit, LightShift™

Supplier: Thermo Fisher Scientific

LightShift™ Chemiluminescent EMSA Kit is a robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterise protein-DNA binding interactions.

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Lambda DNA phage, Hind III digest

Supplier: Rockland Immunochemicals

Lambda DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease Hind III.

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DNA ladder, O'RangeRuler™

DNA ladder, O'RangeRuler™

Supplier: Thermo Fisher Scientific

O'RangeRuler™ DNA ladders are step ladders that consist of basic unit repeats of 10, 15, 20, 50, 100, 200, or 500 bp. The ladders have a higher density of bands at a specific DNA size range and are therefore suitable for more accurate sizing of DNA in agarose or non denaturing polyacrylamide gels.

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Lambda DNA/HindIII Marker

Lambda DNA/HindIII Marker

Supplier: Thermo Fisher Scientific

Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

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High molecular weight transfer buffer (5X)

Supplier: G-Biosciences

High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.

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Substrate for western blotting, 1-Step™ NBT/BCIP

Substrate for western blotting, 1-Step™ NBT/BCIP

Supplier: Thermo Fisher Scientific

The 1-Step NBT/BCIP is a one-component sensitive precipitating alkaline phosphatase substrate for immunoblotting, immunohistochemistry, and in situ hybridisation applications.

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NS/K Fluorogenic substrate kit, QuantaBlu™, Pierce™

Supplier: Thermo Fisher Scientific

QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.

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CN/DAB substrate kit, Pierce™

CN/DAB substrate kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.

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Substrate for western blotting, 1-Step™ NBT/BCIP plus suppressor

Substrate for western blotting, 1-Step™ NBT/BCIP plus suppressor

Supplier: Thermo Fisher Scientific

The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and in situ hybridisation applications.

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Western blotting detection reagents, ECL western blotting substrate

Western blotting detection reagents, ECL western blotting substrate

Supplier: Thermo Fisher Scientific

ECL™ Western blotting substrate is an entry-level Western blotting substrate.

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Pierce™ Chemiluminescent Nucleic Acid Detection Module Kit

Pierce™ Chemiluminescent Nucleic Acid Detection Module Kit

Supplier: Thermo Fisher Scientific

The Pierce™ Chemiluminescent nucleic acid detection module is a complete system for the detection of biotin-labeled nucleic acids for various blotting applications, including Northern/Southern blots, ribonuclease protection assays (RPAs), and electrophoretic mobility shift assays (EMSAs) or gel-shift assays.

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West HisProbe kit, SuperSignal™

West HisProbe kit, SuperSignal™

Supplier: Thermo Fisher Scientific

SuperSignal™ West HisProbe Kit contains HisProbe-HRP conjugate and reagents required for detection of polyhistidine-tagged protein by Western blotting. HisProbe-HRP Conjugate is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.

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DAB substrate kit, metal enhanced, Pierce™

DAB substrate kit, metal enhanced, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Metal enhanced DAB substrate kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense colour for immunohistochemical staining.

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Destaining bags, ethidium bromide

Supplier: Thermo Fisher Scientific

Ethidium bromide destaining bags are used to safely remove ethidium bromide from nucleotide solutions and gels during overnight treatment. Each bag extracts up to 5 mg of Ethidium Bromide from solution, thus minimising the exposure of research personnel to this material.

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SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto

SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto

Supplier: Thermo Fisher Scientific

The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

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Saponin permeating solution, 0,5% w/v solution in PBS (10X)

Supplier: Thermo Fisher Scientific

Saponin permeating solution, is used as a cell permeabilising agent.

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Coomassie Brilliant Blue Destaining Solution, G-Biosciences

Coomassie Brilliant Blue Destaining Solution, G-Biosciences

Supplier: G-Biosciences

After Coomassie Brilliant Blue staining process, the band intensity may be further enhanced by de-staining the stained gel in our CBB De-Staining Solution.

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Multicolour protein ladder, broad range, Spectra™

Supplier: Thermo Fisher Scientific

The Spectra™ Multicolour Broad Range Protein Ladder is a 4-colour protein standard containing 10 prestained proteins (10 to 260 kDa) for use in gel electrophoresis and Western blotting.

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ReadiUse™ 100 bp DNA Ladder

ReadiUse™ 100 bp DNA Ladder

Supplier: AAT BIOQUEST

ReadiUse™ 100 bp DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading.

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