You searched for: Electrophoresis Reagents
Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Western blotting kits, SuperSignal™ west pico chemiluminescent substrate kits, Pierce™
Supplier: Thermo Fisher Scientific
These kits include SuperSignal™ West Pico Substrate that is a highly sensitive enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. The kits also contain reagents for the specific detection of biotinylated antibodies, rabbit IgG or mouse IgG. The substrate’s intense signal output enables detection of picogram amounts of antigen. The sensitivity, intensity and duration of the signal allow for easy detection of HRP using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the immunodetection reagents and reprobed.
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Pierce™ OPD (o-phenylenediamine Dihydrochloride)
Supplier: Thermo Fisher Scientific
OPD (o-phenylenediamine dihydrochloride) is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492 nm by ELISA plate readers.
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Lambda DNA phage, E. coli W8850
Supplier: Rockland Immunochemicals
Lambda phage consists of a virus particle including a head (also known as a capsid), a tail and tail fibres. The head contains the phage's double-stranded circular DNA genome. The phage particle recognises and binds to its host E.coli causing DNA in the head of the phage to be ejected through the tail into the cytoplasm of the bacterial cell. Lambda phage DNA is commonly used as a DNA molecular weight marker. Digestion of the lambda phage genome with HindIII produces a set of 8 fragments consisting of 125, 560, 2000, 2300, 4300, 9400, and 23000 base pairs.
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Vásárolja meg Acros & Alfa Aesar vegyszereit az Avantor®-tól, hogy optimalizálja laboratóriuma ellátását
Már bízik a Thermo Fisher kiváló minőségű vegyi anyagaiban, valamint az Avantor® kiváló szolgáltatásában és támogatásában. Nos, miért ne kombinálná a kettőt?
DNA ladder, low range, MassRuler
Supplier: Thermo Fisher Scientific
MassRuler Low Range DNA Ladder, ready to use, 80 to 1031 bp, is specially designed for accurate quantification and sizing of DNA fragments by agarose gel electrophoresis.
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DNA ladders, ultra low range, FastRuler
Supplier: Thermo Fisher Scientific
FastRuler ultra low range DNA ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10 to 20 mm) after an 8 to 14 minutes run on the appropriate agarose gel.
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RNA ladder, high range, RiboRuler™
Supplier: Thermo Fisher Scientific
RiboRuler™ high range RNA ladder allows precise sizing of RNA molecules in denaturing agarose gels and Northern blotting.
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1-Step™, Pierce™ Chloronaphthol
Supplier: Thermo Fisher Scientific
Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.
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1-Step™ Ultra TMB-Blotting solution, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.
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Ethidium bromide Greenbag™ disposal kit, MP Biomedicals
Supplier: MP Biomedicals
The EtBr Greenbag™ Disposal Kit allows rapid and trouble-free concentration of ethdium bromide from large volumes of solutions into a small 'teabag' containing activated carbon. The Greenbag™ is then conveniently disposed of along with other solid hazardous wastes.
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Amplite® West ECL HRP Substrate *Femto Sensitivity*
Supplier: AAT BIOQUEST
Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.
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Protein immunodetection reagents, SuperSignal™ West Dura extended duration substrate
Supplier: Thermo Fisher Scientific
The SuperSignal™ West Dura Extended Duration Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Dura Substrate enables mid-femtogram detection of antigen by oxidising luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualised on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions.
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Chemiluminescent EMSA kit, LightShift™
Supplier: Thermo Fisher Scientific
LightShift™ Chemiluminescent EMSA Kit is a robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterise protein-DNA binding interactions.
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Lambda DNA phage, Hind III digest
Supplier: Rockland Immunochemicals
Lambda DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease Hind III.
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DNA ladder, O'RangeRuler™
Supplier: Thermo Fisher Scientific
O'RangeRuler™ DNA ladders are step ladders that consist of basic unit repeats of 10, 15, 20, 50, 100, 200, or 500 bp. The ladders have a higher density of bands at a specific DNA size range and are therefore suitable for more accurate sizing of DNA in agarose or non denaturing polyacrylamide gels.
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Lambda DNA/HindIII Marker
Supplier: Thermo Fisher Scientific
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.
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Substrate for western blotting, 1-Step™ NBT/BCIP plus suppressor
Supplier: Thermo Fisher Scientific
The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and in situ hybridisation applications.
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CN/DAB substrate kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.
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NS/K Fluorogenic substrate kit, QuantaBlu™, Pierce™
Supplier: Thermo Fisher Scientific
QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.
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Substrate for western blotting, 1-Step™ NBT/BCIP
Supplier: Thermo Fisher Scientific
The 1-Step NBT/BCIP is a one-component sensitive precipitating alkaline phosphatase substrate for immunoblotting, immunohistochemistry, and in situ hybridisation applications.
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High molecular weight transfer buffer (5X)
Supplier: G-Biosciences
High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.
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Western blotting detection reagents, ECL western blotting substrate
Supplier: Thermo Fisher Scientific
ECL™ Western blotting substrate is an entry-level Western blotting substrate.
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DAB substrate kit, metal enhanced, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Metal enhanced DAB substrate kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense colour for immunohistochemical staining.
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Pierce™ Chemiluminescent Nucleic Acid Detection Module Kit
Supplier: Thermo Fisher Scientific
The Pierce™ Chemiluminescent nucleic acid detection module is a complete system for the detection of biotin-labeled nucleic acids for various blotting applications, including Northern/Southern blots, ribonuclease protection assays (RPAs), and electrophoretic mobility shift assays (EMSAs) or gel-shift assays.
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West HisProbe kit, SuperSignal™
Supplier: Thermo Fisher Scientific
SuperSignal™ West HisProbe Kit contains HisProbe-HRP conjugate and reagents required for detection of polyhistidine-tagged protein by Western blotting. HisProbe-HRP Conjugate is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.
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SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto
Supplier: Thermo Fisher Scientific
The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.
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Destaining bags, ethidium bromide
Supplier: Thermo Fisher Scientific
Ethidium bromide destaining bags are used to safely remove ethidium bromide from nucleotide solutions and gels during overnight treatment. Each bag extracts up to 5 mg of Ethidium Bromide from solution, thus minimising the exposure of research personnel to this material.
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Saponin permeating solution, 0,5% w/v solution in PBS (10X)
Supplier: Thermo Fisher Scientific
Saponin permeating solution, is used as a cell permeabilising agent.
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Multicolour protein ladder, broad range, Spectra™
Supplier: Thermo Fisher Scientific
The Spectra™ Multicolour Broad Range Protein Ladder is a 4-colour protein standard containing 10 prestained proteins (10 to 260 kDa) for use in gel electrophoresis and Western blotting.
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DNA Ladders in TE buffer
Supplier: Biotium
DNA ladders in TE buffer are used for sizing linear double-stranded DNA fragments separated by gel electrophoresis. The 1 kb DNA ladder is suitable for sizing DNA fragments from 250 bp to 10 kb, while the 100 bp DNA ladder is suitable for sizing DNA fragments from 100 bp to 1500 bp.