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289 results for Protein Purification

 

Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

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Ion exchange chromatography media, CM Sepharose™ Fast Flow

Ion exchange chromatography media, CM Sepharose™ Fast Flow

Supplier: Cytiva

CM Sepharose™ Fast Flow is a weak cation exchanger based on the well established Sepharose™ Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow

Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow

Supplier: Cytiva

IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.

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Gel filtration media, Sephadex™ G-15

Gel filtration media, Sephadex™ G-15

Supplier: Cytiva

Sephadex™ G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.

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Affinity chromatography media, IgG Sepharose™ 6 Fast Flow

Affinity chromatography media, IgG Sepharose™ 6 Fast Flow

Supplier: Cytiva

lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose 6 Fast Flow matrix, with human IgG covalently coupled to it.

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Ion exchange chromatography media, SP Sepharose™ High Performance

Ion exchange chromatography media, SP Sepharose™ High Performance

Supplier: Cytiva

SP Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups.

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Ion exchange chromatography media, IEX Sepharose™ Fast Flow

Ion exchange chromatography media, IEX Sepharose™ Fast Flow

Supplier: Cytiva

Q Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades.

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Sepharose™ 4B Gel Filtration Base Matrix

Sepharose™ 4B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ 4B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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Gel filtration media, Sephadex™ G-50

Gel filtration media, Sephadex™ G-50

Supplier: Cytiva

Sephadex™ G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

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Sepharose™ 4 FF Octyl Fast Flow Hydrophobic Interaction Chromatography Media

Sepharose™ 4 FF Octyl Fast Flow Hydrophobic Interaction Chromatography Media

Supplier: Cytiva

Octyl Sepharose™ 4 Fast Flow resin is based on cross-linked 4% agarose. The aliphatic ligand is immobilised to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.

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Affinity chromatography media, Streptavidin Sepharose™ High Performance

Supplier: Cytiva

Streptavidin Sepharose™ High Performance is an affinity chromatography resin designed for fast and reliable, high resolution purification of biotinylated biomolecules.

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Q Sepharose™ XL Ion Exchange Chromatography Media, Cytiva

Q Sepharose™ XL Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

Q Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose Fast Flow media.

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Sepharose™ CL-4B Octyl Hydrophobic Interaction Chromatography Media

Supplier: Cytiva

Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.

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Affinity coupling media, EAH Sepharose™ 4B

Affinity coupling media, EAH Sepharose™ 4B

Supplier: Cytiva

EAH Sepharose™ 4B allows for the stable carbodiimide-based coupling of carboxyl groups to Sepharose® 4B via an 11-atom hydrophilic spacer arm that permits extremely stable coupling.

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Ion exchange chromatography media, SOURCE™ 30S

Ion exchange chromatography media, SOURCE™ 30S

Supplier: Cytiva

SOURCE 30S is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix It is modified with sulphonate (S) strong cation exchange groups.

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Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow

Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow

Supplier: Cytiva

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.

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Sepharose™ 6B Gel Filtration Base Matrix

Sepharose™ 6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ 6B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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Calmodulin Sepharose™ 4B Affinity Chromatography Media, Cytiva

Calmodulin Sepharose™ 4B Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Calmodulin Sepharose™ 4B is calmodulin immobilised by the CNBr method to Sepharose™ 4B. Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells. This protein is involved in many cellular processes such as glycogen metabolism, cytoskeletal control, neurotransmission, phosphate activity and control of NAD⁺/NADP⁺.

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Blue Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva

Blue Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Blue Sepharose™ 6 Fast Flow is cibacron blue 3G coupled to Sepharose™ 6 Fast Flow. It is well established as an adsorbent for albumin and interferon at both laboratory and process scale. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.

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Protein A from Staphylococcus aureus, MP Biomedicals

Supplier: MP Biomedicals

Protein A is a recombinant Fc-binding protein from Staphylococcus aureus, Cowan strain-1 gene. The product is a carboxy truncated material containing 301 amino acid residues. The non-binding regions have been removed to minimise potenial steric interference.

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Biacore consumables and accessories

Biacore consumables and accessories

Supplier: Cytiva

Biacore maintenance kits are convenient kits for proper instrument maintenance.

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Butyl Sepharose™ High Performance Hydrophobic interaction chromatography media

Butyl Sepharose™ High Performance Hydrophobic interaction chromatography media

Supplier: Cytiva

Butyl Sepharose™ HP media is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.

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Affinity chromatography media, Blue Sepharose™ High Performance

Supplier: Cytiva

This media is suitable for affinity chromatography.

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Phenyl Sepharose™ 6 Fast Flow (High Sub) Hydrophobic Interaction Chromatography Media

Phenyl Sepharose™ 6 Fast Flow (High Sub) Hydrophobic Interaction Chromatography Media

Supplier: Cytiva

Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity

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Affinity chromatography media, Lentil Lectin Sepharose™ 4B

Supplier: Cytiva

Lentil lectin is a metalloprotein, containing Ca₂₊ and Mn₂₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars.

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Ion exchange chromatography media, ANX Sepharose™ 4 Fast Flow (High Sub)

Ion exchange chromatography media, ANX Sepharose™ 4 Fast Flow (High Sub)

Supplier: Cytiva

ANX Sepharose™ 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers.

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Ion exchange chromatography media, Q Sepharose™ High Performance

Ion exchange chromatography media, Q Sepharose™ High Performance

Supplier: Cytiva

Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.

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DEAE Sephadex™ A-25 Ion Exchange Chromatography Media, Cytiva

DEAE Sephadex™ A-25 Ion Exchange Chromatography Media, Cytiva

Supplier: Cytiva

DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

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Concentrator

Supplier: G-Biosciences

The concentrator is a high molecular weight polymer which will not migrate across the dialysis membrane. The concentrator is placed against the dialysis membrane of the Tube-O-DIALYZER™, where it rapidly absorbs water from the sample and reduces the sample volume. The concentrator can be used with dialysis bags and other dialysis devices.

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Accessories and spare parts for ÄKTA start
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Affinity purification media, GammaBind™ Plus Sepharose™

Supplier: Cytiva

GammaBind™ Plus Sepharose™ is GammaBind G, type 3, covalently immobilised to Sepharose™ CL-6B by malimide linkage.

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