You searched for: Protein Purification

Supplier: Thermo Fisher Scientific

Protein A UltraLink™ and Protein A Plus UltraLink™ resins are extremely durable beaded polyacrylamide supports to which IgG-binding Protein A has been immobilised for use in IgG purification methods. Protein A resin is used to bind and purify IgG in gravity-flow, spin-column or medium pressure chromatography methods.

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Ni-NTA Superflow Agarose is a nitrilotriacetic acid modified Superflow 6 support charged with divalent nickel (Ni⁺²) used for FPLC purification of poly-histidine-tagged proteins.

Supplier: Thermo Fisher Scientific

Pierce™ chicken IgY purification kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction.

Supplier: Thermo Fisher Scientific

Pierce™ Protein G UltraLink™ and Protein G Plus UltraLink™ resins are useful for purification of antibodies and immunoprecipitation (IP). Samples containing IgG are incubated with the resin-immobilised Protein G in a buffer that facilitates binding. After non-IgG and non-antigen components of the sample are washed from the resin, the bound IgG and antigen may be recovered by elution.

Supplier: Thermo Fisher Scientific

The Pierce™ NAb™ Spin Kits are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilised protein resin enables quick purification of 100 to 1000 µg of IgG from 25 to 500 µl of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.

Supplier: Thermo Fisher Scientific

AminoLink™ coupling resin is crosslinked, 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilisation of antibodies and other proteins through primary amines.

Supplier: Thermo Fisher Scientific

The Pierce™ Protein Refolding Kit includes high-purity reagents and detailed instructions for using a matrix strategy to determine optimal buffer conditions for refolding recombinant proteins that have been denatured and solubilised from inclusion bodies.

Supplier: Thermo Fisher Scientific

Pierce™ High capacity streptavidin agarose consists of purified recombinant streptavidin that has been covalently immobilised at a high density onto high-quality crosslinked 6% beaded agarose.

Supplier: Thermo Fisher Scientific

AminoLink™ Immobilisation Kit contains AminoLink™ coupling resin and associated reagents sufficient to perform 5 protein immobilisations, with an expected yield of 2 to 20 mg protein per column.

Supplier: Thermo Fisher Scientific

Pierce SDS-PAGE sample prep kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.

Supplier: Thermo Fisher Scientific

The HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Supplier: Thermo Fisher Scientific

Pierce™ F(ab')2 micro preparation kit enables efficient Fab generation from IgG.

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein L can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Supplier: Thermo Fisher Scientific

Pierce™ MM(PEG)_n reagents are methyl-terminated PEG compounds activated with a maleimide group for covalent pegylation of sulfhydryls on proteins or assay surfaces.

Supplier: Thermo Fisher Scientific

Pierce™ Fab preparation kits use immobilised papain protease to digest human or mouse IgG antibodies to make separate Fab and Fc fragments and subsequently to purify the Fab using Protein A agarose.

Supplier: Thermo Fisher Scientific

UltraLink™ Hydrazide resin is used to prepare affinity columns with antibodies or other glycolsylated proteins.

Supplier: 3M

3M™ Polisher ST is a fully encapsulated anion exchange (AEX) product to reduce host sell protein impurities, viruses, and other negatively charged contaminants in flow-through polishing chromatography of biopharmaceutical process streams.

Supplier: Thermo Fisher Scientific

The Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kits use immobilised ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments.

Supplier: Thermo Fisher Scientific

Pierce™ HA-Tag IP/Co-IP kit provides the affinity resin, positive control and other reagents necessary to perform immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) reactions with an HA-tagged bait protein.

Supplier: Thermo Fisher Scientific

CarboxyLink™ immobilisation kit is for covalent immobilisation of peptides or other carboxyl-containing (—COOH) molecules to a porous, beaded resin for use in affinity purification procedures.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Maleimide Activated NeutrAvidin™ Protein is a specially-prepared form of avidin that decreases background in biotin-binding.

Supplier: Thermo Fisher Scientific

The Pierce™ Magnetic ChIP Kit provides a convenient method for efficient isolation of chromatin-bound DNA by immunoprecipitation for subsequent quantitation by PCR.

Supplier: Thermo Fisher Scientific

Pierce™ Monomeric avidin agarose is immobilised onto 4% beaded agarose for purifying biotinylated proteins, peptides and other molecules.

Supplier: Thermo Fisher Scientific

The Melon™ Gel Spin Plate Kit for IgG Screening offers a high throughput format for quick purification and screening of antibodies.

Supplier: Thermo Fisher Scientific

Pierce™ Avidin agarose is purified avidin immobilised on crosslinked 6% beaded agarose for the affinity purification of biotinylated macromolecules.

Supplier: Thermo Fisher Scientific

Immobilised TCEP disulphide Reducing Gel eliminates the need to use laborious and inefficient gel filtration methods to separate the reduced sample from the reducing agent.

Supplier: Thermo Fisher Scientific

The Pierce™ Sulfhydryl Addition Kit contains all necessary components to introduce free sulfhydryl groups into any primary amine-containing molecule, purify the modified molecule and quantify the added sulfhydryl groups.

Supplier: Thermo Fisher Scientific

The Pierce™ Classic Magnetic IP/Co-IP Kit enables highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of antigens and protein-complexes using less than 10 µg of antibody and a magnetic separator.

Supplier: Thermo Fisher Scientific

Pierce™ conjugate purification kit is designed to eliminate excess, unconjugated HRP or alkaline phosphatase enzyme from labeled antibodies (IgG) after performing antibody-enzyme conjugation reactions. The single-use column-kit is intended for purification of 0,5 to 50 mg of antibody conjugate dissolved in tris-buffered saline.