You searched for: Cellular Assays

Supplier: AAT BIOQUEST

This Live or Dead™ Cell Viability Assay Kit uses two fluorescent indicators: Cellbrite™ Red (Ex/Em = 613/631 nm) for labelling viable cells and a cell-impermeable DNA-binding dye Nuclear Blue™ DCS1 (Ex/Em = 360/450 nm) for labelling dead cells with damaged membranes.

Supplier: AAT BIOQUEST

The detection of intracellular dihydronicotinamide adenine dinucleotide NADH and its phosphate ester NADPH is important for disease diagnostics and drug discovery.

Supplier: OZ BIOSCIENCES

MTT cell proliferation assay kit is designed for spectrophotometric quantification of cell growth, viability and proliferation and can be used as a direct indicator of cytotoxicity (such as for screening anticancer drugs) and apoptosis.

Supplier: AAT BIOQUEST

SO Green™ 520WS Singlet Oxygen Sensor has been developed as a water-soluble fluorescent probe for detecting extracellular singlet oxygen since it is cell-impermeant.

Supplier: OZ BIOSCIENCES

ROS Detection Assay Kit is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2', 7'-Dichlorodihydrofluorescein diacetate (DCF-DA). Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidised by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell.

Supplier: AAT BIOQUEST

The endoplasmic reticulum (ER) is a type of organelle in the cells of eukaryotic organisms that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae.

Supplier: G-Biosciences

CytoScan™ LDH Cytotoxicity assay quantitatively measures the cytosolic, lactate dehydrogenase (LDH) enzyme, which is released from damaged cells. The released LDH is measured with a coupled enzymatic reaction that results in the conversion of a tetrazolium salt (iodonitrotetrazolium; (INT)) into a red color formazan by diaphorase. The resulting formazan is measured at 490 nm.

Supplier: AAT BIOQUEST

Detecting and imaging RNA molecules in living cells is extremely important for a wide variety of molecular biology procedures including physical transportation, interpretation of genetic information, regulation of gene expression and some essential bio-catalytic roles.

Supplier: AAT BIOQUEST

Cell Meter™ Live Cell No Wash Caspase 3/7 Imaging Kit uses our recently developed cell-permeable fluorogenic caspase substrate, ApoSight™ Green Caspase 3/7, the first fluorogenic probe, for the direct detection of caspase activities in live cells.

Supplier: AAT BIOQUEST

Although Lissamine Rhodamine B chloride is one of the most popular labeling reagents of sulfonyl chloride, it is quite unstable in water. Even in anhydrous DMF, Lissamine Rhodamine B chloride tends to give a very complicated reaction mixture. It reacts with thiols, alcohols, phenols, aliphatic amines and aromatic amines indiscriminatingly. LRB Red™ is a succinimidyl ester and has the spectral properties identical to those of Lissamine Rhodamine B chloride. LRB Red™ is a superior replacement for Lissamine Rhodamine B chloride. LRB Red™ only reacts with aliphatic amines such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable. Compared to Lissamine Rhodamine B chloride, LRB Red™ has much higher labeling efficiency, and more importantly the resulted conjugates are much easier to be purified due to its much cleaner reactions. We strongly recommend that you consider using LRB Red™ to replace Lissamine Rhodamine B chloride for labeling peptides and oligonucleotides.

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. Amplite® fluorimetric sodium ion quantification kit uses our robust sodium ion indicator dye, SoNa™ 520, which exhibit great fluorescence intensity enhancement upon binding to sodium ions.

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. Amplite® fluorimetric lithium ion quantification kit uses our new robust lithium-ion indicator dye, Lithiumighty™ 520, which exhibits great fluorescence intensity enhancement upon binding to lithium ions.

Supplier: Sartorius

Incucyte® Nuclight rapid red dye for live-cell nuclear labelling was formulated explicitly for cell labelling of living mammalian cells. The dye is a cell-permeable DNA strain that specifically stains nuclei in cells and is ideally suited to the mix-and-read, real-time quantitation.

Supplier: Biosensis

Amylo-Glo RTD reagent is designed to stain amyloid plaques in tissue sections.

Supplier: Biotium

This kit is designed to measure Renilla luciferase activity in transfected cell reporter gene assays.

Supplier: Cayman Chemical

Nrf2 Transcription Factor Assay kit is a 96-well assay for measurement of Nrf2.

Supplier: Biotium

Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.

Supplier: Biosensis

Amylo-Glo RTD reagent is designed to stain amyloid plaques in tissue sections.

Supplier: G-Biosciences

AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change colour in response to the chemical reduction of growth medium due to cell growth.

Supplier: Biotium

Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry or microscopy.

Supplier: Biotium

CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. They are unique among membrane stains in which they can withstand both fixation and detergent permeabilisation.

Supplier: Biotium

A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.

Supplier: Biotium

The Yeast Viability Staining Kit is a fluorescent assay to determine yeast viability. Each kit contains two fluorescent components: CF® Dye-labeled Concanavalin A (Con A) and Live-or-Dye™ viability dye. Biotium offers 3 different color combinations to choose from.

Supplier: Biotium

The Yeast Live-or-Dye™ Fixable Live/Dead Staining Kit is a fixable, fluorescent assay to determine yeast viability. The kit contains two fluorescent components, Thiazole Orange and Live-or-Dye™ 568/583.

Supplier: Biotium

A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.

Supplier: Biotium

Live-or-Dye NucFix™ Red is a red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected by flow cytometry or fluorescence microscopy.

Supplier: Biotium

This kit is designed to measure Firefly and Renilla luciferase activities in transfected cell reporter assays. Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalising transfection control for firefly luciferase assays.

Supplier: Thermo Fisher Scientific

BCA Protein Assay Kit can be used to measure very dilute protein concentrations in very small sample volumes. The kit accurately measures protein concentrations from 0,5 to 30 μg/ml in tube assays and 1 to 20 μg/ml in 96- or 384-well plate assays. It is used for studying protein:protein interactions, measuring column fractions after affinity chromatography, estimating percent recovery of membrane proteins from cell extracts and high-throughput screening of fusion proteins.

Supplier: Abnova

Antioxidant Assay Kit is a quantitative colorimetric determination of total antioxidant capacity of cellular samples.

Supplier: Abnova

Malachite Green Phosphate Assay Kit provides a fast, reproducible, and non-radioactive method for measuring inorganic free phosphate in aqueous solution.