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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Non-animal protein blocking Agent, NAP-BLOCKER™

Non-animal protein blocking Agent, NAP-BLOCKER™

Supplier: G-Biosciences

NAP-BLOCKER™ is an animal-free protein blocking agent recommended for improved assay sensitivity, minimal non specific binding and a high signal-to-background ratio. It is suitable for Western blots, dot blots and assay development.

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Buffer, HEPES solution pH 7,5 (1 mol/L)

Supplier: PanReac AppliChem

HEPES buffer solution pH 7,5 (1 mol/l)

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RBB Buffers

Supplier: OMEGA BIO-TEK

RBB Buffer, 1000 ml, Omega Bio-Tek

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Pierce™ Blocking Buffers, Protein Free

Pierce™ Blocking Buffers, Protein Free

Supplier: Thermo Fisher Scientific

These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.

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Buffer, HEPES solution pH 8 (1 mol/L)

Supplier: PanReac AppliChem

HEPES buffer solution pH 8 (1 mol/l)

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Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™

Dulbecco's phosphate buffered saline (DPBS), for cell culture, powder format, HyClone™

Supplier: HyClone products (Cytiva)

Balanced salt solution used for multiple cell culture applications (powder form).

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Blocker™ Blocking Buffer, Casein

Blocker™ Blocking Buffer, Casein

Supplier: Thermo Fisher Scientific

Blocker™ Casein in PBS or TBS are ready-to-use, solutions of purified casein protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

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10X Citrate Buffer, pH 6,0

10X Citrate Buffer, pH 6,0

Supplier: AAT BIOQUEST

A citrate buffer is an aqueous solution composed of a weak acid (citric acid) and its conjugate base (sodium citrate).

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CytoWatch™ Multiplexing Buffer for Flow Cytometry, 20X

CytoWatch™ Multiplexing Buffer for Flow Cytometry, 20X

Supplier: AAT BIOQUEST

CytoWatch™ Multiplexing Buffer is a buffer optimised for the multicolor flow cytometric analysis of cells.

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Cell culture buffers, Hank's balanced salt solutions (HBSS) HyClone™

Cell culture buffers, Hank's balanced salt solutions (HBSS) HyClone™

Supplier: HyClone products (Cytiva)

Balanced salt solutions for cell culture applications.

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In situ hybridisation wash reagent

In situ hybridisation wash reagent

Supplier: ENZO LIFE SCIENCES

Hybridisation wash reagent provides a high quality hybridisation wash solution for in situ hybridisation analyses using DNA probes labeled with biotin, digoxigenin or fluorescein.

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Pierce™ Fish Serum Blocking Buffer,Thermo Scientific

Supplier: Thermo Fisher Scientific

This Fish Serum blocking buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.

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Blocking buffers, protein-free, Pierce™

Blocking buffers, protein-free, Pierce™

Supplier: Thermo Fisher Scientific

Pierce Protein-free blocking buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.

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Tris-Glycine buffer

Supplier: EDVOTEK

For vertical polyacrylamide gel electrophoresis.

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IP lysis buffer, Pierce™

IP lysis buffer, Pierce™

Supplier: Thermo Fisher Scientific

The IP lysis buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS.

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Extraction buffers, FOCUS™

Extraction buffers, FOCUS™

Supplier: G-Biosciences

FOCUS™ Extraction Buffers are experimentally optimised concentrations of critical agents, buffering and stabilising agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulphobetaines (SB). They are designed to produce optimal protein extraction and improved spot resolution for 2D gel analysis.

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Protein blocking agent, BLOT-QuickBlocker™

Protein blocking agent, BLOT-QuickBlocker™

Supplier: G-Biosciences

BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.

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Wash buffers, femto TBST™ and femto PBST™

Wash buffers, femto TBST™ and femto PBST™

Supplier: G-Biosciences

The femto TBST™ and femto PBST™ wash buffers will enhance sensitivity of immunoassays by minimising the 'washing out' of immuno agents or immuno complexes, a common problem associated with classical TBST and PBST buffers. The wash buffers aid in the generation of cleaner backgrounds, resulting in a larger ratio of signal to background. These can be used for incubation and washing procedures.

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Protein solubilisation buffer

Protein solubilisation buffer

Supplier: G-Biosciences

FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.

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Phosphate-buffered saline (PBS), Corning®

Phosphate-buffered saline (PBS), Corning®

Supplier: Corning

Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate

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Buffer, TBE (Tris-borate-EDTA) powder for 10X concentrated solution

Supplier: PanReac AppliChem

Powder for 10X concentrated aqueous solution (170,28 g/L). Composed of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂.

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Western Blot Transfer buffer, 10X, methanol-free, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Methanol-Free 10X Western Blot Transfer Buffer is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) western blotting transfer apparatus.

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VWR®, Eluent buffers for amino acid analyzer

VWR®, Eluent buffers for amino acid analyzer

Supplier: VWR Chemicals

Regenerating F, Li

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Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer

Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer

Supplier: Thermo Fisher Scientific

Phenol-chloroform, is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6,7 or pH 8,0.

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ReadiUse™ Cell Detaching Buffer (50 ml)

ReadiUse™ Cell Detaching Buffer (50 ml)

Supplier: AAT BIOQUEST

ReadiUse™ cell detaching buffer is a cell detachment solution that does not contain mammalian or bacterial-derived products.

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ReadiUse™ Yeast Cell Lysis Buffer, 5X

ReadiUse™ Yeast Cell Lysis Buffer, 5X

Supplier: AAT BIOQUEST

ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.

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Dulbecco's phosphate-buffered saline (DPBS), Corning®

Dulbecco's phosphate-buffered saline (DPBS), Corning®

Supplier: Corning

Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7,0 to 7,6

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Pierce TMT™ Elution Buffer

Pierce TMT™ Elution Buffer

Supplier: Thermo Fisher Scientific

Thermo Scientific TMT Elution Buffer is for use with Thermo Scientific Immobilized Anti-TMT Resin to enrich samples for peptides that have been chemically labeled with Tandem Mass Tag™ (TMT™) Reagents.

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Binding and elution buffers for antibody purification, Pierce™

Binding and elution buffers for antibody purification, Pierce™

Supplier: Thermo Fisher Scientific

These buffers ensure high-yield, non denaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

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ReadiUse™ Annexin Binding Buffer, Optimised for Imaging and Flow Cytometry

ReadiUse™ Annexin Binding Buffer, Optimised for Imaging and Flow Cytometry

Supplier: AAT BIOQUEST

The ReadiUse™ Annexin Binding Buffer *Optimised for Imaging and Flow Cytometry* is an isotonic buffer designed to promote the labeling of apoptotic cells with annexin V conjugates.

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