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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: G-Biosciences

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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VWR® PBS/Tween® 20, 20X, Blot Wash Buffer, UltraPure Grade

VWR® PBS/Tween® 20, 20X, Blot Wash Buffer, UltraPure Grade

Supplier: VWR Chemicals

PBS Tween® 20 blot wash buffer is a 20X concentration with 1% Tween® 20.

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VWR® AVS TITRINORM, Cesium chloride/lanthanum chloride buffer solution acc. to schinkel for atomic absorption spectroscopy (aas)

VWR® AVS TITRINORM, Cesium chloride/lanthanum chloride buffer solution acc. to schinkel for atomic absorption spectroscopy (aas)

Supplier: VWR Chemicals

This material is intended for use as an ionisation buffer for AAS

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Buffer, EDTA buffer solution pH 8,5 (0,5 mol/L)

Supplier: G-Biosciences

A DNase and RNase free solution of 0.5M EDTA.

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10X Tris-EDTA buffer pH 9,0

Supplier: Novus Biologicals

10X Tris-EDTA buffer pH 9.0.

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VWR® Tris Buffer Solution, pH 7,4 (0,1 mol/L) for Biotechnology

VWR® Tris Buffer Solution, pH 7,4 (0,1 mol/L) for Biotechnology

Supplier: VWR Chemicals

Cell culture buffer, TRIS buffer solution pH 7,4 (0,1 mol/l)

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Fixation buffer for microscopy and flow cytometry

Fixation buffer for microscopy and flow cytometry

Supplier: Biotium

Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.

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Dry buffer packs, Carbonate-Bicarbonate Buffer, JAW™

Supplier: G-Biosciences

Premixed buffer powders that simply require adding deionised water to generate a ready-to-use buffer.

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Buffer HF-Improved

Supplier: Thermo Fisher Scientific

Buffer used for pretreatment of planar silicon devices when plating with nickel plating solution products 44069 and 44070.

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Buffer kit for calibration of fluorescent Ca² indicators

Supplier: Biotium

Kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca² indicators (1,2).

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Caspase cell lysis buffer

Supplier: ENZO LIFE SCIENCES

Caspase cell lysis buffer, Enzo Life Sciences

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VWR® Phosphate Buffered Saline (PBS) with 0,05% Tween®20

VWR® Phosphate Buffered Saline (PBS) with 0,05% Tween®20

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) with 0,05% Tween® 20, pH 7,5 is a ready-to-use 1× concentration.

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GET buffer

Supplier: Rockland Immunochemicals

GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.

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VWR® Sodium Acetate Buffer 3,0 M Solution, pH 5,2

VWR® Sodium Acetate Buffer 3,0 M Solution, pH 5,2

Supplier: VWR Chemicals

With diethyl pyrocarbonate (DEPC).

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Buffers, potassium phosphate buffer (0,5 mol/L) solutions

Supplier: Cayman Chemical

Potassium phosphate buffer solution (0,5 mol/l), pH value: 7,0

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6X GelRed prestain loading buffer with tracking dye

Supplier: Biotium

6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).

    
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VWR® Phosphate Buffered Saline (PBS), Low Phosphate, 10X

VWR® Phosphate Buffered Saline (PBS), Low Phosphate, 10X

Supplier: VWR Chemicals

Irradiated concentrate of PBS, suitable for use in washing cells after dilution.

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RIPA cell lysis buffer 2

Supplier: ENZO LIFE SCIENCES

RIPA cell lysis buffer 2, Enzo Life Sciences

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Blocking Buffer 10X

Blocking Buffer 10X

Supplier: Abcam

Blocking Buffer 10X

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Wash buffer salts, lyophilised, Enzo Life Sciences

Supplier: ENZO LIFE SCIENCES

Wash Buffer Salts can be used in tissue preparations, immunohistochemical staining procedures and in assays that require HRP-linked detection reagents.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Rockland Immunochemicals

This product was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

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VWR® AVS TITRINORM, Cesium chloride/aluminium nitrate buffer solution acc. to schuhknecht and schinkel for determination of na, k and li by flame aas
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Caspase assay buffer

Supplier: ENZO LIFE SCIENCES

50 mM HEPES, pH 7,4, 100 mM NaCl, 0,1% CHAPS, 10 mM DTT, 1 mM EDTA, 10% glycerol.

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Buffer, PBS tablets

Buffer, PBS tablets

Supplier: PanReac AppliChem

PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.

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Tris-Borate-EDTA (TBE) buffer, 10X

Supplier: PanReac AppliChem

10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.

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VWR®, Phosphate buffered saline (PBS) 1X, for biotechnology

VWR®, Phosphate buffered saline (PBS) 1X, for biotechnology

Supplier: VWR Chemicals

137 mM sodium chloride, 2,7 mM potassium chloride, 12 mM phosphate buffer

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Competent cell buffer

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7,5.

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VWR®, Hybridisation buffers with 50% formamide

VWR®, Hybridisation buffers with 50% formamide

Supplier: VWR Chemicals

Hybridisation cocktail offering blocking and hybridisation for optimised complimentary base pairing of DNA and RNA in Southern blot, Northern blot, dot blot, colony and in situ hybridisation.

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Cell lysis buffer 4, (100 ml), Enzo Life Sciences

Supplier: ENZO LIFE SCIENCES

Cell lysis buffer 4, Enzo Life Sciences

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Blocking buffer, fish gelatin

Supplier: Biotium

Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

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