You searched for: Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Buffers, concentrated solutions, Fluka™
Supplier: Honeywell Chemicals
pH Buffer, concentrate, pH value: 1,00, ±0.02, Ampoule
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Buffer solution for chlorine determination
Supplier: Hach
For determination of chlorine by amperometric titration. APHA formulation 100 ml bottle.
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Phosphate buffer solution pH 7,00 (20 °C), Fluka™
Supplier: Honeywell Chemicals
Potassium dihydrogen phosphate / di-sodium hydrogen phosphate
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Vásárolja meg Acros & Alfa Aesar vegyszereit az Avantor®-tól, hogy optimalizálja laboratóriuma ellátását
Már bízik a Thermo Fisher kiváló minőségű vegyi anyagaiban, valamint az Avantor® kiváló szolgáltatásában és támogatásában. Nos, miért ne kombinálná a kettőt?
Sodium acetate, Corning® 3M™
Supplier: Corning
Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.
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EDTA solution, 0,5 M, molecular biology grade, Calbiochem®, Millipore®
Supplier: Merck Millipore (Calbiochem)
Sterile-filtered solution of 0,5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC). Used in molecular biology applications.
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Buffer, sodium acetate, 3M aq, soln,, pH 4,5, autoclaved
Supplier: Thermo Fisher Scientific
Sodium acetate, 3M aq, solution, pH 4,5, autoclaved
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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins
Supplier: Thermo Fisher Scientific
Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific
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Buffer solution pH 7, Phosphate buffer
Supplier: Thermo Fisher Scientific
Potassium dihydrogen phosphate and di-Sodium hydrogen phosphate in aqueous solution, Phosphate, pH value: 7,00, ±0,02
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Solubilisation buffer, 2D-Xtract™
Supplier: G-Biosciences
A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.
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Buffer, sodium acetate, 3 M aq, soln,, pH 7,0, RNAse free
Supplier: Thermo Fisher Scientific
Sodium acetate, 3M aq, solution, pH 7,0, RNAse free, Liquid
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Buffer, sodium acetate, 3 M aq, soln,, pH 7,0, autoclaved
Supplier: Thermo Fisher Scientific
Sodium acetate, 3M aq, solution, pH 7,0, autoclaved
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TISAB III solution (Total Ionic Strength Adjustment Buffer) for fluoride determinations
Supplier: Mettler - Toledo
TISAB III solution for fluoride determinations
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Buffers, BIS-TRIS propane, 0,2 M solutions
Supplier: Thermo Fisher Scientific
2,2'-(Propane-1,3-diyldiimino)bis[2-(hydroxy methyl)propane-1,3-diol]
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Buffer, sodium acetate, 1M aq, soln,, pH 4,5, RNAse free
Supplier: Thermo Fisher Scientific
Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid
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Ionisation buffer solutions (CRMs), for AAS and ICP, Fluka™
Supplier: Honeywell Chemicals
Matrix Modifier, 2% Potassium (K) in 1% HCl 1%
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Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.
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Buffer solutions, colour coded, certified (20 °C), Fluka™
Supplier: Honeywell Chemicals
Borate, pH value: 10.00, 9.988 - 10.012, Reference temperature: 20 °C, Blue
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Protein extraction reagent, BugBuster® 10X, Millipore®
Supplier: NOVAGENE
BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.
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T-PER™ Tissue Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.
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EDTA, 0.5M pH 8.0, Corning®
Supplier: Corning
EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity. The use of EDTA therefore allows for greater control over many experiments.
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10% SDS (Sodium Dodecyl Sulfate), Corning®
Supplier: Corning
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.
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Tris-Glycine (TG) buffer,, MP Biomedicals
Supplier: MP Biomedicals
Tris-glycine buffer is used for western blotting and gel electrophoresis.
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Buffer, imidazole buffer solution pH 7,5 (2 mol/L)
Supplier: G-Biosciences
Imidazole buffer solution pH 7,5 (2 mol/l)
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GST buffer kit
Supplier: Cytiva
GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.
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Buffer, PBS solution 10X concentrate (phosphate buffered saline), MP Biomedicals
Supplier: MP Biomedicals
Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
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Tris Borate EDTA buffer, for molecular biology, 10x, DNAse, RNAse and Protease free, pH 8.3
Supplier: Thermo Fisher Scientific
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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IPG buffers
Supplier: Cytiva
IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.
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2-D Protein Extraction Buffer
Supplier: Cytiva
Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.
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His buffer kit
Supplier: Cytiva
His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.
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Ab buffer kit
Supplier: Cytiva
Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.