You searched for: Assays

Supplier: ENZO LIFE SCIENCES

Cellular assay, Color-de-Lys™ developer concentrate, 300 µl

Supplier: ENZO LIFE SCIENCES

Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.

Supplier: ENZO LIFE SCIENCES

ER-ID® Red assay kit (GFP-CERTIFIED®) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.

Supplier: ENZO LIFE SCIENCES

The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.

Supplier: ENZO LIFE SCIENCES

For the detection of Cytochrome c translocation from mitochondria into cytosol during apoptosis.

Supplier: ENZO LIFE SCIENCES

Cellular assay, Cell counting kit-8

Supplier: ENZO LIFE SCIENCES

This kit has been designed for monitoring protein stability under systematic thermal stress conditions.

Supplier: ENZO LIFE SCIENCES

The Granzyme B Assay Kit for Drug Discovery is a complete assay system designed to measure the protease activity of granzyme B. It contains a colorimetric substrate (IEPD-pNA) to measure this activity; cleavage of the p-nitroanilide (pNA) group from the colorimetric substrate increases absorption at 405 nm.

Supplier: G-Biosciences

The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.

Supplier: ENZO LIFE SCIENCES

COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic "comet" shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA.

Supplier: ENZO LIFE SCIENCES

The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.

Supplier: ENZO LIFE SCIENCES

Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VDVAD-AFC.

Supplier: Biotium

Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.

Supplier: Cayman Chemical

Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

Supplier: G-Biosciences

CB™ Protein Assay, a Coomassie dye based protein assay based on the Bradford Protein Assay, is suitable for the simple and rapid estimation of protein concentration. The binding of protein to the dye results in a colour change from brown to blue. The change in colour density is proportional to protein concentration. Protein estimation can be performed using as little as 0,5 µg protein.

Supplier: Cayman Chemical

HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.

Supplier: ENZO LIFE SCIENCES

The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered in vivo.

Supplier: Cayman Chemical

Measure superoxide and hydrogen peroxide levels in living cells.

Supplier: Biotium

EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.

Supplier: ENZO LIFE SCIENCES

The p70 S6K activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

Supplier: ENZO LIFE SCIENCES

The assay is designed to measure either MDA alone (in hydrochloric acid) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid). It is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole with MDA and 4-hydroxyalkenals to yield a stable chromophore with maximal absorbance at 586 nm.

Supplier: ENZO LIFE SCIENCES

Calcium dye

Supplier: ENZO LIFE SCIENCES

BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation. It is suitable for applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase. It is most widely applied to protein phosphatase assays.

Supplier: ENZO LIFE SCIENCES

The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.

Supplier: G-Biosciences

The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labelled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased. The fluorescence of the FITC-labeled peptide is measured with excitation at 485 nm and emission at 530 nm to determine protease activity. The kit detects picogram level of proteases present in the sample.

Supplier: ENZO LIFE SCIENCES

Cellular assay, Fluor-de-Lys®-green substrate, in 50 mM solution in DMSO

Supplier: ENZO LIFE SCIENCES

The SIRT5 Fluorometric Drug Discovery Kit is a sensitive fluorometric assay is designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5.

Supplier: ENZO LIFE SCIENCES

The PROTEOSTAT® PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT® PDI detection dye.

Supplier: ENZO LIFE SCIENCES

The HDAC fluorometric cellular activity assay allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.

Supplier: Abcam

Sensitive, reproducible assay for initial screening of potential glutaminase inhibitors.