You searched for: Nucleic Acid Purification Kits and Reagents

Supplier: OMEGA BIO-TEK

The Mag-Bind® Bacterial DNA kit allows rapid and reliable isolation of high quality genomic DNA (gDNA) from a wide variety of bacterial species. Up to 0,5 ml gram positive or gram negative bacterial culture can be processed each time. The key to the system are the Mag-Bind® particles that avidly, but reversibly, bind DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed.

Supplier: ENZO LIFE SCIENCES

EPIXTRACT® DNA isolation kit provides a simple method to isolate DNA from plasma, serum, and body fluids.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Universal Pathogen DNA 96 Kit allows rapid and reliable isolation of high-quality pathogen and host genomic DNA from tissue samples. Up to a 30 mg tissue sample is recommended and can be processed in less than 60 minutes. The system allows for automation after sample lysis via Hamilton STAR™, Thermo KingFisher™ Flex, Applied Biosystems® MagMAX™, Qiagen Biosprint, and other liquid handling instruments.

Supplier: OMEGA BIO-TEK

The E-Z 96® Plant DNA DS Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridisation applications.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Plant DNA DS 96 Kit is designed for difficult samples (DS) which are hard to lyse or contain high amounts of polysaccharides and polyphenols. The system uses a CTAB-based lysis buffer which does not require organic solvents. The proprietary binding system prevents inhibitors from binding to the magnetic beads. The wash buffer system further removes polysaccharides, phenolic compounds and enzyme inhibitors from plant tissue lysate. An optional rebinding step is included with the kit but most samples do not require the additional treatment.

Supplier: OMEGA BIO-TEK

Isolate host genomic, bacterial, fungal spore, and viral DNA and viral RNA from tissue, urine, serum, and fecal samples using magnetic beads.

Supplier: ENZO LIFE SCIENCES

PCR/Gel Clean Up Kits are quick and simple kits for purifying DNA from PCR, gels and labelling reactions for a variety of downstream applications.

Supplier: OMEGA BIO-TEK

Automation-ready, prefilled 96-well plates for quick and convenient DNA extraction from blood, saliva, cultured cells, and swabs for magnetic processors.

Supplier: OMEGA BIO-TEK

Sequentially isolate DNA and RNA in two separate eluates from the same FFPE sample.

Supplier: OMEGA BIO-TEK

Purify endotoxin free (<0,1 EU/µg) plasmid DNA using magnetic beads from up to 50 ml culture volume.

Supplier: OMEGA BIO-TEK

Modified from the E.Z.N.A.® Plant RNA kit, the E.Z.N.A.® Fungal RNA kit uses an improved buffer system that is more efficient for fungal samples. This kit provides convenient and rapid methods for the isolation of total RNA from a variety of fungal species. Rather, the method involves a simple and rapid precipitation step for removal of much of the polysaccharides and phenolic compounds commonly found in fungal tissues. In combination with HiBind® RNA spin columns, this method permits purification of high quality RNA from as much as 200 mg tissue. The system is also efficient enough to purify total RNA from as little as 10 mg of tissue or 100 cells. Purified RNA can be used for many downstream applications such as RT-PCR, northern blotting, Poly(A)+ mRNA purification, and differential display.

Supplier: OMEGA BIO-TEK

Selected protease digestion releases microgram amounts of RNA from FFPE samples. The purified nucleic acids, although highly fragmented, are suitable for a variety of downstream genomic and gene expression analyses. E.Z.N.A® spin column-based kits are ideal for low throughput applications while Mag-Bind® magnetic beads-based kits are designed specifically for high throughput users with automation capability.

Supplier: Corning

The AxyPrep™ Mag PCR Normalizer Kit utilizes a paramagnetic bead-based purification system for PCR clean up.

Supplier: OMEGA BIO-TEK

Gel purification of DNA is a common technique used for the isolation of specific DNA fragments. However, most methods either fail to completely remove agarose (which can lead to problems in downstream manipulations), shear the DNA, or result in very low yields.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Blood DNA HV Kit CE IVD is designed for rapid and reliable isolation of high-quality genomic DNA from up to 4 ml whole blood samples.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridisation applications.

Supplier: Corning

The AxyPrep™ FragmentSelect-I Kit is optimized for Illumina next generation sequencing platforms and Life Technologies (SOLiD) fragment size selection needs to simplify the library construction workflow. The kit is designed for fragment size selection needs in the Roche/454’s DNA Genome sequencer library construction workflow.

Supplier: OMEGA BIO-TEK

Hi-Bind® DNA mini columns are silica glass fibre columns that can bind up to 100 μg of genomic DNA or 35 μg of plasmid DNA per prep. Columns feature an attached lid and a vacuum luer for processing with vacuum manifolds and by centrifugation.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Stool DNA 96 Kit allows rapid and reliable isolation of high-quality genomic DNA (both pathogen and host DNA) from stool samples.

Supplier: OMEGA BIO-TEK

Mag-Bind® Blood and Tissue DNA Kit CE IVD offers a versatile method for the isolation of high-quality DNA from a wide variety of samples including fresh or frozen animal cultured cells and tissues, up to 250 µl whole blood, buccal swabs, up to 500 µl saliva, and dried blood spots.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® soil DNA kit is formulated to isolate high purity cellular DNA from soil samples typically containing humic acid and inhibitors of PCR. This kit has been successfully used to isolate DNA from tough-to-lyse bacteria, fungi, and algae that inhabit a range of samples including clay, sandy, peaty, chalky, and loamy soil samples. Isolated DNA can be used for most downstream applications including PCR, southern blotting, and SNP analysis.

Supplier: OMEGA BIO-TEK

Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid kits integrate an efficient endotoxin removal step into the plasmid purification procedure to produce high quality transfection grade plasmid.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30 µg of high-quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA purification is simplified with HiBind® Mini Column technology into three quick steps: Bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing. E.Z.N.A.® Plasmid Mini Kit I is available in either V-spin or Q-spin column format. V-spin column formats have an attached lid while Q-spin columns do not. Both versions can be used for centrifugation or vacuum protocols.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Viral RNA Kit is designed for the isolation of viral RNA from cell free fluids such as plasma, serum, urine and cell culture supernatant. The procedure completely removes contaminants and enzyme inhibitors making viral RNA isolation fast, convenient and reliable. The kit is also suitable for the isolation of total RNA from cultured cells, tissues and bacteria. RNA purified using the E.Z.N.A.® Viral RNA method is ready for all downstream applications such as RT-PCR.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Forensic DNA Kit is designed to provide a rapid and easy method for the isolation of genomic DNA from forensic samples such as dry blood, buccal swabs, and semen samples for consistent PCR and Southern analysis. This kit can also be used for the preparation of genomic DNA from mouse tail snips, whole blood, buffy coat, serum, and plasma.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Bacterial RNA kit is designed for isolation of high-quality total RNA from variety of bacterial strains. Up to 1×10⁹ log-phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridisation techniques.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Plasmid Maxi Kit is the maxi scale system to deliver high-quality plasmid DNA using the spin column format, without costly accessories. The HiBind® DNA Maxi column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50 to 200 ml of overnight culture in LB medium typically produces 0,5 to 1 mg of high copy number plasmid DNA. Up to 500 ml of culture may be processed when working with low copy number plasmid. The product is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, transfection of mammalian cells, transcription invitro, and other applications.

Supplier: OMEGA BIO-TEK

Selected protease digestion releases microgram amounts of DNA from FFPE samples.

Supplier: OMEGA BIO-TEK

The MicroElute RNA clean-up kit is designed to purify and concentrate RNA (from 50 μg down to picogram amounts) from enzymatic reactions or for desalting RNA samples (e.g. following phenol extraction and ethanol precipitation). The procedure uses lower elution volumes, making it well suited for applications require highly concentrated RNA sample.

Supplier: Corning

Removing excess dye terminator is an essential step prior to Sanger sequencing. Carryover of the excess dye into the sequencing reactions may result in dye blobs resulting in inaccurate results. The AxyPrep™ Mag DyeClean kit utilises a paramagnetic bead-based purification system for Sanger sequencing reaction dye terminator clean-up. The protocol is simple and comprises binding, washing and elution steps which can be performed directly in the thermal cycling plate. This kit requires no centrifugation or filtration steps.