Ön a következőre keresett: qPCR/RT-qPCR Enzymes and Kits

Supplier: Quantabio

A cDNS szintézis és a PCR amplifikáció ugyanabban a tesztcsőben történhet, anélkül hogy az edény fel kellene nyitni a két lépés között. A készletek maximális RT-PCR hatékonyságra és specificitásra vannak optimalizálva, lehetővé téve az alacsony példányszámú kópiák torzítatlan koamplifikálását magasabb példányszámú referenciagének jelenlétében. Emiatt a qScript™ egylépéses qRT-PCR készletek érzékenyebbek a kereskedelemben kapható más qPCR reagenseknél. A qScript™ egylépéses mesterkeverék maximálja mind a reverz transzkriptáz és a PCR enzimek aktivitását, miközben minimálisra csökkenti a primer-dimer képződést. A nagy specificitás az AccuStart™ Taq DNS polimeráznak köszönhető , amely monoklonális antitestekhez kötődik a kiváló hot starthoz . A 95 °C-ra történő felfűtésnél az antitestek irreverzibilisen denaturálódnak, teljes aktivitású és módosulatlan Taq DNS polimerázt szabadítva fel. A qScript™ egylépéses qRT-PCR készletek sorozatában megtalálható olyan típus, amely kompatibilis a kereskedelmi forgalomban lévő real-time PCR készülékekkel, beleértve azokat is, amelyek ROX referenciafestékes normálást igényelnek.

Supplier: Thermo Fisher Scientific

The Verso™ qRT-PCR system combines the Verso™ RT enzyme with ABsolute™ Blue qPCR master mix to provide maximum convenience and versatility. Available in a 1- or 2-step format, as well as multiple pack sizes, to suit all application and throughput requirements.

Supplier: Thermo Fisher Scientific

The Verso™ RT-PCR system combines a reverse transcription enzyme, improved priming options and optimised buffers to generate high yield and full length cDNA. With a variety of kit options for both 1- and 2-step RT-PCR reactions, Verso™ provides optimum flexibility and sensitivity in an easy to use format.

Supplier: VWR Chemicals

VWR® One-Step RT-qPCR Kit is designed to facilitate rapid, sensitive, and precise detection and quantification of various RNA sequences via dye-based qPCR. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: VWR Chemicals

VWR® Probe One-Step RT-qPCR Kit is designed to facilitate rapid, sensitive, and precise detection and quantification of various RNA sequences via hydrolysis probes. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: Quantabio

PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

Supplier: VWR Chemicals

Real-Time PCR kit for detection and quantification of Salmonella spp. in food samples. Kit that contains all necessary reagents for 100 reactions. This product provides rapid and efficient detection and quantification of Salmonella spp.

Supplier: VWR Chemicals

Real-Time PCR kit for detection and quantification of Legionella spp. in environmental samples (ISO/TS 12869). Kit contains all necessary reagents for 100 reactions. This product provides rapid and efficient detection and quantification of Legionella spp.

Supplier: VWR Chemicals

VWR® qPCR Master Mix features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: VWR Chemicals

This kit is designed to detect the pathogen microorganism Listeria monocytogenes in food samples, after enrichment steps, according to standard microbiology procedures. It can also be used to confirm suspect colonies isolated in selective culture media.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and dye-based qPCR in a single tube.

Supplier: VWR Chemicals

VWR® Probe One-Step RT-qPCR Master Mix with UDG is designed for the detection and quantitation of RNA targets and is an ideal choice for multiplex applications. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: VWR Chemicals

VWR® Probe qPCR Master Mix features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: Quantabio

Superior sensitivity for viral RNA detection.

Supplier: Quantabio

PerfeCTa® Multiplex qPCR SuperMix, low ROX is a 2X concentrated, ready to use reaction cocktail that contains all components, except primers, probe(s), and template for real time qPCR on Applied Biosystems 7500 or Stratagene MX series of real time PCR systems.

Supplier: Quantabio

Qscript lyo 1-step is a lyophilised single-reaction reagent optimised for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes.

Supplier: Quantabio

A Perfecta® qPCR SuperMix 2X koncentrált, használatra kész reakciókeverékek, amelyek a primereken, a próbán és a qPCR templáton kívül minden komponenst tartalmaznak.

Supplier: Quantabio

No compromises. Unparalleled performance meets sustainability.

Supplier: Quantabio

Ezek a FastMix reagensek pufferek és a stabilizálók segítségével, a maximális PCR hatékonyságra, reprodukálhatóságra, és - a TaqMan® és TaqMan® MGB próbás kémiai rendszerekkel - a maximális érzékenységre vannak optimizálva. A PerfeCTa® qPCR FastMix termékek egyik kulcskomponense az AccuFast™ Taq DNS polimeráz, amely azonnali aktiválást és gyors polimerizálást tesz lehetővé. A hővel való aktiválásnál (20 másodperc, 95 °C-on) az antitestek irreverzibilisen denaturálódnak, teljesen aktív, módosítatlan Taq DNS polimerázt felszabadítva. Ez specifikus és hatásos primer-extenziót tesz lehetővé, a reakció szobahőmérsékleten való összeállításának kényelme mellett. A FastMix reagensek maximális hatékonyságot, érzékenységet, specificitást és erőteljes fluoreszcens jelet adnak, akár gyors, akár hagymányos ciklusú körülmények között.

Supplier: Quantabio

PerfeCTa® qPCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues or animal tissues. It is a versatile and robust Real-Time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR ToughMix® contains all required reaction components, except primers, probe(s) and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

Supplier: Quantabio

qScript™ XLT One-Step RT-qPCR ToughMix® is a ready to use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: Quantabio

A SuperMix 2X koncentrált, használatra kész reakciókeverék real time qPCR-hez, amely a primereken, a próbákon és a templátokon kívül, minden komponenst tartalmaz. A rendszer meghaladja a hagyományos PCR mesterkeverékek korlátait, mivel lehetővé teszi egy tesztcsőben maximum öt célszekvencia torzítatlan amplifikálását. Gyakori probléma a multiplex PCR esetén, hogy az alacsony példányszámban keletkezett kópiáknak nagy a referenciatargetek általi szuppressziója. Ez eltorzítja vagy elfedi az alacsony példányszámú célszekvenciák megjelenését és mennyiségi meghatározását. A PerfeCTa® MultiPlex qPCR SuperMix olyan dinamikus tartományt és érzékenységet biztosít a multiplex qPCR esetén, ami összemérhető az egyszeres qPCR próbás mérésekével, anélkül, hogy szüksége lenne a primer koncentrációk korlátozására vagy változtatására.

Supplier: Biotium

A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: Quantabio

PerfeCTa® MultiPlex qPCR ToughMix® is a 5X concentrated, ready to use reaction cocktail for Real-Time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix® allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCta® MultiPlex qPCR ToughMix® has been optimised to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols.

Supplier: Biotium

EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.