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Enzymes

Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.

Cholera Toxin (Vibrio cholerae) G Protein Activator

Supplier: Enzo Life Sciences

G protein activator.

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D-Glucose dehydrogenase

Supplier: Thermo Fisher Scientific

Applications: Diagnostics and cofactor regeneration of NAD(P)H in reductive processes.

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Human recombinant Matriptase

Supplier: Enzo Life Sciences

Recombinant human matriptase (aa 596-855) fused at the N-terminus to a His-tag.

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Human recombinant Guanylyl cyclase alpha1beta1 (soluble) (from Sf9 cells)

Supplier: Enzo Life Sciences

Produced in Sf9 cells. Fused at the C-terminus to a His-tag.

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Human recombinant Matriptase 2

Supplier: Enzo Life Sciences

Produced in Drosophila Schneider 2 (S2) cells. Recombinant human matriptase-2 (aa 78-811) is fused at the C-terminus to a V5-His-tag.

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Mg²⁺/ATP Activating Solution

Supplier: Enzo Life Sciences

This solution contains pre-coupled Mg-ATP for immediate use as an energy source for a range of ATP-dependent reactions (e.g. ubiquitin thioester conjugation, proteasomal degradation, SUMO conjugation).

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Human Recombinant Aldehyde Dehydrogenase 1-A2 (from E. coli)

Supplier: ProSci Inc.

Aldehyde dehydrogenase 1 family member A2 (ALDH1A2), also known as retinaldehyde dehydrogenase 2 (RALDH2), belongs to the aldehyde dehydrogenase family which contains two members, the ALDH1 s (ALDH1A1, ALDH1A2 and ALDH1A3) and the 9-cis retinaldehyde dehydrogenase ALDH8 s.

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Pancreatin

Supplier: PanReac AppliChem

Pancreatin

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Recombinant uridine phosphorylase Salmonella typhimurium

Supplier: Apollo Scientific

Recombinant uridine phosphorylase Salmonella typhimurium

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Human recombinant Caspase-4 (from E. coli)

Supplier: Enzo Life Sciences

Produced in E. coli.

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Human recombinant Caspase-8 (from E. coli)

Supplier: Enzo Life Sciences

Produced in E. coli.

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Human recombinant Caspase-8 (from E. coli)

Supplier: Enzo Life Sciences

Produced in E. coli.

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Human recombinant Caspase-3 (from E. coli)

Human recombinant Caspase-3 (from E. coli)

Supplier: Enzo Life Sciences

Produced in E. coli.

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Apis mellifera Phospholipase A2

Supplier: Enzo Life Sciences

Isolated from Apis mellifera.

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T4 GP32 Protein (from E.coli)

Supplier: G-Biosciences

T4 GP32 is a single-stranded DNA binding protein with key role in DNA replication, recombination and repair. T4 GP32 binds cooperatively to single stranded DNA and stimulates cell-free DNA synthesis directed by the T4-encoded DNA polymerase. It improves the efficiency of reverse transcriptase during RT-PCR as well as increases the yield of PCR products.

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Sheep Cyclooxygenase (from Seminal vesicles)

Supplier: Enzo Life Sciences

Isolated from ram seminal vesicles.

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Human recombinant Cu/Zn superoxide dismutase (from E. coli)

Supplier: Enzo Life Sciences

Produced in E. coli.

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Human recombinant Caspase-9 (from E. coli)

Supplier: Enzo Life Sciences

Produced in E. coli. C-terminal Human caspase-9 (aa 131-416).

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Human recombinant T cell protein tyrosine phosphatase (from E. coli)

Supplier: Enzo Life Sciences

Produced in E. coli. T-cell phosphatase with a C-terminal 11 kDa deletion (TCdeltaC11) (aa 1-317).

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Proteinase K solution

Proteinase K solution

Supplier: PanReac AppliChem

Proteinase K is used to destruct proteins in cell lysates (tissue, cultured cells) and to liberate nucleic acids, since it very effectively digests DNases and RNases.

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Dnase

Supplier: OMEGA BIO-TEK

For DNase digestion during RNA purification. The RNase-free DNase set provides a rapid and efficient on-column digestion of DNA during RNA purification from a wide variety of sample sources using E.Z.N.A.® RNA kits. The DNases can be efficiently removed in subsequent steps. Generally, DNase digestion is not required for RNA purified with E.Z.N.A.® RNA kits since the silica-membrance, spin-column technology efficiently removes the majority of the DNA without DNase treatment. However, complete DNA removal may be necessary for certain RNA applications that are sensitive to very small amounts of DNA.

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Peroxidase, Horseradish, RZ3 (POD, HRP, HRPO)

Supplier: US Biological

Horseradish Peroxidase (HRP) is a hemoprotein catalysing the oxidation by hydrogen peroxide of a number of substrates such as ascorbate, ferrocyanide, cytochrome C and the leuko form of many dyes.

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Deoxyribonuclease I

Supplier: Rockland Immunochemicals

Deoxyribonuclease I (usually called DNase I), is an endonuclease coded by the human gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin.

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Bovine MSG-Trypsin™

Bovine MSG-Trypsin™

Supplier: G-Biosciences

Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.

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Human Recombinant EN-TEV Protease (from E. coli)

Human Recombinant EN-TEV Protease (from E. coli)

Supplier: Abcam

Recombinant EN-TEV Protease protein is a Tobacco etch virus Fragment protein, in the 2038 to 2279 aa range, expressed in Escherichia coli, with >=95% purity and suitable for SDS-PAGE, Size Exclusion Chromatography.

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Nuclease S1

Supplier: Rockland Immunochemicals

Nuclease S1 is an endonuclease that is active against single-stranded DNA and RNA molecules. Although its primary substrate is single-stranded, it can also introduce single-stranded breaks in double-stranded DNA-RNA or DNA-RNA hybrids. Nuclease S1 is used in the laboratory as a reagent in nuclease protection assays. It is used in removing single stranded tails from DNA molecules to create blunt ended molecules and opening hairpin loops generated during synthesis of double stranded cDNA.

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Ribonuclease A

Supplier: PanReac AppliChem

Ribonuclease A

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Human recombinant Matrix Metalloproteinase-7

Supplier: Apollo Scientific

Human recombinant Matrix Metalloproteinase-7

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Recombinant disulfide bond isomerase

Supplier: Apollo Scientific

Recombinant disulfide bond isomerase

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Human recombinant Caspase-1 (from E. coli)

Supplier: Enzo Life Sciences

Produced in E. coli.

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