Supplier: STEMCELL Technologies

Mouse monoclonal IgG1, antibody against human, chimpanzee CD4, PE-conjugated.

Supplier: STEMCELL Technologies

Hamster (Armenian) monoclonal IgG, antibody against mouse, CD152 (CTLA-4), PE-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2b, antibody against human, chimpanzee, CD45RA, PE-conjugated.

Supplier: STEMCELL Technologies

SARS-CoV-2 Recombinant Spike Protein, aa319-541 is expressed in Pichia pastoris and is one of four structural proteins encoded by the SARS-CoV-2 genome. The Spike Protein plays a key role in attachment to host cells allowing invasion through clathrin-mediated endocytosis. The spike protein can be cleaved by host cell proteases after aa685 to yield the N-terminal S1 subunit, and C-terminal S2 region. The S1 subunit is responsible for interacting with the host cell receptor (angiotensin-converting enzyme II) through a receptor-binding domain that is highly conserved with SARS-CoV. The S1 subunit has two conformations: a ‘down’ conformation in which the receptor is inaccessible, and an ‘up’ conformation in which the receptor is accessible. These conformational changes are key for monoclonal antibody drugs and vaccine development. At the amino terminus of the polypeptide chain, SARS-CoV-2 Recombinant spike protein contains a polyhistidine tag and a SUMOstar site.

Supplier: STEMCELL Technologies

Human Recombinant Angiotensin-Converting Enzyme 2 (ACE2) Protein, aa18-740, is expressed in HEK293 cells. ACE2 activity results in vasodilation by degrading vasoconstrictor angiotensin II to produce vasodilator angiotensin. Decreased expression of this enzyme is associated with cardiovascular disease and possibly also glomerular injury in diabetic nephropathy. ACE2 contains a peptidase domain at the N-terminus and a single transmembrane helix. ACE2 is also the primary human receptor for the surface spike glycoprotein of SARS-CoV-2 and SARS-CoV. This receptor is expressed in human lung and small intestine epithelia as well as the heart, kidney, and testes. The peptidase domain of ACE2 binds to the protein-binding domain of the S1 protein subunit of the spike protein; this binding results in a cleavage site becoming exposed. Cleavage, or ‘protein priming’, is done by TMPRSS2, a cellular serine protease, and by cathepsins. This cleavage activates the S2 subunit of the spike protein, allowing the fusion of the viral membrane to the host cell membrane. At the carboxy terminus, Human Recombinant ACE2 Protein contains a TEV site and a human IgG1 Fc tag.

Supplier: STEMCELL Technologies

FRET-based peptide substrate for detection of protease activity of coronaviruses - 100 tests.

Supplier: STEMCELL Technologies

Ready-to-use marker dye for loading DNA samples into agarose gel wells and tracking migration during electrophoresis.

Supplier: STEMCELL Technologies

Support cell attachment, growth, and proliferation with CellAdhere™ Type I Collagen, Human, Lyophilised. The most abundant type of collagen in the human body, type I collagen is a natural extracellular matrix protein that provides structural support and mimics physiological conditions, making it an ideal cultureware coating surface to support cell attachment in 2D applications.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched non-human primate cells

Supplier: STEMCELL Technologies

Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.

Supplier: STEMCELL Technologies

Serum-free neurophysiological basal medium for improved neuronal function.

Supplier: STEMCELL Technologies

Stimulating T cells with HHV8 (K8.1) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HHV8 (K8.1) Peptide pool is a lyophilised mixture of 55 peptides from glycoprotein K8.1 of human herpesvirus 8 (HHV-8) and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 228 on K8.1. Expressed in the late-lytic phase, K8.1 is a viral envelope protein that binds to heparin sulfate on the cell surface and facilitates HHV 8 infection (Zoeteweij et al; Birkmann et al.; Wang et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with CMV (IE-2) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. CMV (IE-2) Peptide pool is a lyophilised mixture of 143 peptides from immediate early protein 2 (IE2) of human cytomegalovirus (CMV; strain AD169), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 580 on IE2. IE2 is critical for viral infection and for the expression of CMV early genes (Marchini et al.). It also controls transcription initiation for a subset of early-late and late viral promoters via RNA polymerase II (Li et al.). IE2, along with IE1, may contribute to the survival of CMV-infected cells by blocking tumor necrosis factor alpha (TNF-α)-induced apoptosis (Zhu et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Scale up manual cell isolations from large-volume samples, such as leukopaks and whole blood. Rather than splitting cell suspensions and performing multiple rounds of isolations, the Easy 250 magnet can process up to 225 ml and 1.25×10¹⁰ cells in a single isolation.

Supplier: STEMCELL Technologies

The 96-Well PCR microplate magnet is designed for magnetic bead-based nucleic acid extraction and is compatible with EasySep™ Total nucleic acid extraction kit.

Supplier: STEMCELL Technologies

Ensure standardised freezing - without the use of alcohol - for most cell types with Corning® CoolCell® LX Cell freezing containers.

Supplier: STEMCELL Technologies

A member of the apolipoprotein family, Apolipoprotein A-I (apo A-I) is a major glycoprotein component of high density lipoprotein (HDL) particles (Frank and Marcel) which facilitate the reverse transport of cholesterol from peripheral tissues to the liver. Apo A-I binds to the scavenger receptor SR-A1, allowing HDL particles to interact with cells in peripheral tissues (Neyen et al.), and is involved in the esterification of cholesterol, acting as a cofactor for lecithin cholesterol acyltransferase (LCAT; Frohlich). Apo A-I has been shown to bind to the scavenger receptor SR-B1 (Williams et al.) and the beta chain of ATP synthase (Martinez et al.) on hepatocytes, highlighting its importance in HDL endocytosis. Mutations in the APOA1 encoding gene may result in dysregulated HDL levels, leading to an increased risk of amyloidosis and ischemic heart disease (Haase et al.; Obici et al.). This protein product contains a His-residue tag at the amino end of the polypeptide chain. This protein product contains a His-residue tag at the amino end of the polypeptide chain. For consistency and reproducibility across your applications, sclerostin from STEMCELL comes lyophilised with ≥92% purity.

Supplier: STEMCELL Technologies

Adipocyte-specific adhesion molecule (ASAM) may play a role in cell-cell adhesion as it is expressed in epithelial cells of various tissues and is associated with tight junctions (Raschberger et al., Sze et al.). This type 1 transmembrane protein belongs to the cortical thymocyte marker in the Xenopus (CTX) family within the immunoglobulin superfamily. Sequence alignment analysis has shown that ASAM shares conserved characteristics with other CTX-like proteins, including extracellular V-type and C2-type domains, a transmembrane region, and a cytoplasmic tail (Raschperger et al.). ASAM is also expressed in adipocytes, and was found to be upregulated in white adipose tissue (WAT) in a rodent model of obesity and type II diabetes (Eguchi et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, adipocyte-specific adhesion molecule from STEMCELL comes lyophilised with ≥94% purity, and is verified by LAL analysis to ensure endotoxin levels are ≤1,0 EU/μg protein.

Supplier: STEMCELL Technologies

A cytokine belonging to the type 1 interferon family, Interferon beta (IFN beta) binds IFN alpha/beta receptors (IFNAR) that activate tyrosine kinases and initiate the interferon-induced Jak-STAT signaling pathway, which modulates many key immune processes (Smieja et al.). In an experimental model involving cardiac fibroblasts isolated from rats, IFN beta was found to induce both pro- and anti-inflammatory cytokines production by activating different STAT proteins (Bolivar et al.). The anti-inflammatory effects of IFN beta have been studied in the context of autoimmune disorders, and there are currently multiple approved IFN beta drugs for treatment of relapsing forms of multiple sclerosis (Filipi and Jack). IFN beta is produced by immune cells, including macrophages, and non-immune cells, such as fibroblasts and epithelial cells (Ivashkiv and Donalin). The crystal structure of IFN beta shares characteristics with other type I interferons. It comprises five alpha-helices with four of them forming a helix bundle, and one long and three shorter loops connecting the helices (Karpusas et al.). For consistency and reproducibility across your applications, interferon alpha 1 from STEMCELL comes lyophilised with ≥87% purity, specific activity EC50 ≤18 pg/mL, and LAL analysis verification ensuring endotoxin levels are ≤1,0 EU/μg protein.

Supplier: STEMCELL Technologies

Interferon alpha 1 (IFNA1) belongs to the type 1 interferon family of cytokines, which bind interferon alpha receptors (IFNAR; composed of IFNAR1 and IFNAR2 subunits) that are involved in interferon-induced JAK-STAT signaling (Shemesh et al.). Interferons have inhibitory effects on viral replication and pathogenesis, and studies have found that IFN alpha can prevent the spread of herpes simplex virus (HSV) (Mikloska and Cunningham), and human immunodeficiency virus (HIV) (George and Mattapallil). Pegylated forms of IFN alpha are currently approved for the treatment of chronic hepatitis B (Woo et al.). IFN alpha also displays anti-tumor effects by regulating cell growth and proliferation, and it is used in the treatment of different cancers (Tagliaferri et al.). Interferons display alpha-helical structures with four helices forming an antiparallel alpha-helix bundle (Walter). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, interferon alpha 1 from STEMCELL comes lyophilised with ≥90% purity, specific activity EC50 ≤40 to 200 pg/ml, and LAL analysis verification ensuring endotoxin levels are ≤1,0 EU/μg protein.

Supplier: STEMCELL Technologies

Mouse monoclonal antibody against human CD69 (AIM).

Supplier: STEMCELL Technologies

Hamster (Armenian) monoclonal antibody against mouse T cell receptor beta, unconjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal antibody against human TNF-alpha, biotin-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal antibody against human perforin, FITC-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal antibody against human IL-4, APC-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal antibody against human, chimpanzee CD3, APC-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal antibody against mouse CD3, FITC-conjugated.

Supplier: STEMCELL Technologies

cGMP, human T cell activation and expansion reagent.

Supplier: STEMCELL Technologies

Rat monoclonal antibody against mouse CD86 (B7-2), PE-conjugated.

Supplier: STEMCELL Technologies

Immunodensity isolation of untouched human cells.