2429 Résultats pour : "STEMCELL Technologies"

Supplier: STEMCELL Technologies

Rat monoclonal IgG1, kappa isotype control antibody, unconjugated.

Supplier: STEMCELL Technologies

Thrombopoietin (TPO) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. TPO stimulates the proliferation and maturation of megakaryocytes and has an important role in regulating the level of circulating platelets in vivo. TPO also promotes the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multilineage progenitor cells. It is commonly used with other cytokines such as stem cell factor (SCF) and Flt3/Flk-2 Ligand to promote expansion of primitive hematopoietic cells in culture (Hitchcock andamp; Kaushansky). The TPO receptor, c-Mpl, is expressed at all stages of megakaryopoiesis, from hematopoietic stem and progenitor cells to mature platelets.

Supplier: STEMCELL Technologies

Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine that activates NF-kB, MAPK, and PI3K/AKT pathways. Activated T cells and macrophages are the primary producers of TNF-α in response to inflammation and infectious conditions. Many other cell types have been shown to produce TNF-α, among them B cells, NK cells, mast cells, neutrophils, dendritic cells, microglia, endothelial cells, smooth muscle cells, cardiomyocytes, and fibroblasts. TNF-α has cytotoxic effects on cancer cells in vitro by stimulating anti-tumor immuno- suppressive responses. TNF-α stimulates expression of E- and P-selectins, thus facilitating adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells (Zelová and Hosek). Other effects of TNF-α include vasodilatation and edema formation.

Supplier: STEMCELL Technologies

Mouse monoclonal IgM, kappa isotype control antibody, unconjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against human, mouse, cat CD45R (B220), biotin-conjugated.

Supplier: STEMCELL Technologies

Hamster (Armenian) monoclonal IgG1 antibody against mouse CD3e, biotin-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, chimpanzee CD45, Pacific Blue™-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against mouse CD62L (L-selectin), biotin-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against mouse Sca1 (Ly-6A/E), unconjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human CD10, unconjugated.

Supplier: STEMCELL Technologies

Serum-free culture supplement for expansion of human erythroid cells.

Supplier: STEMCELL Technologies

Membrane-permeable fluorescent probe for the detection of live neurons - 0,1 ml.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD83, Alexa Fluor® 488-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2b antibody against human, rhesus HLA-DR, Alexa Fluor® 488-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, chimpanzee CD22, PE­conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against mouse CD4, unconjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against human, mouse, cat CD45R (B220), unconjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgM antibody against mouse CD49b (integrin α2), unconjugated.

Supplier: STEMCELL Technologies

Animal component-free DNase I for digestion of DNA.

Supplier: STEMCELL Technologies

Interleukin 5 (IL-5) is a member of the short-chain 4-α-helical bundle subset of hematopoietic cytokines. It binds to the specific α receptor, which in turn recruits the common β receptor that initiates signaling via JAK/STAT and MAPK pathways. IL-5 is produced by Th2 cells, eosinophils, activated mast cells, and ILC2 cells. IL-5 affects the differentiation, activation, and survival of eosinophils, thus playing an important role in allergic inflammation, asthma, and parasite immunity. Stimulation of eosinophils with IL-5 leads to their activation, upregulation of CD11b expression, and inhibition of apoptosis (Shearer).

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2a antibody against human, mouse, rat beta-tubulin III, Alexa Fluor® 488-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against mouse CD4, PerCP-Cy5.5-conjugated.

Supplier: STEMCELL Technologies

Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine that promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages, including basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes (Yang et al.; Dorssers et al.; Broughton et al.). IL-3 is produced by activated T cells and has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by basophils and eosinophils (Broughton et al.). The IL-3 receptor consists of a unique alpha subunit (CD123) and a beta common subunit (βc or CD131) that is shared with the receptors for IL-5 and GM-CSF, and is the principal signal transduction subunit for these cytokines. IL-3 binding to the heterodimeric receptor activates JAK/STAT, MAPK, and PI3K signaling pathways (Woodcock et al.).

Supplier: STEMCELL Technologies

Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages. In synergy with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al.). GM-CSF was first purified from the culture of mouse lung tissue after lipopolysaccharide treatment. GM-CSF is produced by multiple cell types, including stromal cells, Paneth cells, macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells, fibroblasts, chondrocytes, and Th1 and Th17 cells (Francisco-Cruz et al.). The receptor for GM-CSF (GM-CSFR) is composed of two subunits: the cytokine-specific α subunit (GMRα; CD116) and the common subunit βc (CD131) shared with IL-3 and IL-5 receptors (Broughton et al.). GM-CSFR is expressed on hematopoietic cells, including progenitor cells and immune cells, as well as non-hematopoietic cells. GM-CSF is able to stimulate the development of DCs that ingest, process, and present antigens to the immune system (Francisco-Cruz et al.).

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2b antibody against human OCT4 (OCT3), unconjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2a antibody against mouse NK1.1 (CD161), PerCP-Cy5.5-conjugated.

Supplier: STEMCELL Technologies

Methylcellulose-based medium with EPO for human cells.

Supplier: STEMCELL Technologies

Integrated workflow containing tools to isolate and expand CD34+ progenitor cells from peripheral blood and their subsequent reprogramming to iPS cells.

Supplier: STEMCELL Technologies

Methylcellulose-based medium without cytokines for human cells.

Supplier: STEMCELL Technologies

Serum-free and xeno-free medium for the expansion of human T cells.