146 Results for: "TriLink BioTechnologies"
TriLink® CleanCap® Cas9 Nickase mRNA (5 moU)
Supplier: TriLink BioTechnologies
Cas9 Nickase mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9) that contains a D10A amino acid substitution. This mRNA also contains a C-terminal nuclear localisation signal followed by a HA tag.
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TriLink® CleanScribe™ RNA Polymerase
Supplier: TriLink BioTechnologies
CleanScribe™ RNA Polymerase is a novel enzyme that reduces double-stranded RNA (dsRNA) formation in IVT RNA synthesis.
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TriLink® CleanCap® Cas9 mRNA
Supplier: TriLink BioTechnologies
Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double stranded DNA cleavage.
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TriLink® 5-Methylcytidine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.
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TriLink® 2'-Deoxyadenosine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
TriLink® 2'-Deoxyadenosine-5'-O-(1-Thiotriphosphate)
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TriLink® 2'-Deoxycytidine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
TriLink® 2'-Deoxycytidine-5'-O-(1-Thiotriphosphate)
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TriLink® 2'-Fluoro-2'-deoxycytidine-5'-Triphosphate
Supplier: TriLink BioTechnologies
2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve in vivo stability.
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TriLink® 2'-O-Methyl-N6-Methyladenosine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® 2'-O-Methyl-N6-Methyladenosine-5'-Triphosphate
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TriLink® 2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddATP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.
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TriLink® 5-Propargylamino-2'-deoxyuridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
5-Propargylamino-2'-deoxyuridine can be enzymatically incorporated into DNA and cDNA to introduce primary amine groups. Primary amines can be labeled with NHS ester fluors and haptens for detection and immobilisation of the resulting DNA strand.
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TriLink® 7-Deaza-7-Propargylamino-2'-deoxyguanosine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® 7-Deaza-7-Propargylamino-2'-deoxyguanosine-5'-Triphosphate
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TriLink® 2'-Deoxythymidine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.
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TriLink® Sulf Cyanine5 Aminoallyluridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
Cyanine 5-Aminoallyluridine-5'-Triphosphate is a substrate for T7 RNA polymerase, allowing the incorporation of cyanine 5 into RNA and mRNA during in vitro transcription. Cyanine 5 incorporation allows for fluorescent detection of RNA and mRNA independent of protein translation.
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CleanCap® AU EGFP saRNA
Supplier: TriLink BioTechnologies
This construct expresses an enhanced version of the green fluorescent protein (EGFP), originally isolated from the jellyfish Aequorea victoria, as self-amplifying RNA (saRNA). EGFP is a commonly used direct detection reporter in mammalian cells, yielding bright green fluorescence with an emission peak at 509 nm.
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Biotinylation kits, ChromaLink™ Biotin Antibody Labelling kit
Supplier: TriLink BioTechnologies
ChromaLink™ Biotin Protein Labelling kit offers a complete solution for the quantitative biotinylation of proteins from 20 kDa to 200 kDa and from 25 µg to 1 mg. The kit incorporates traceable biotin on proteins and allows for direct spectrophotometric verification of biotin incorporation. A simple Å280 and Å354 measurement determines the number of biotins per protein molecule.
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Conjugation kits, protein-protein conjugation kit
Supplier: TriLink BioTechnologies
The Protein-Protein Conjugation kit is designed to easily and efficiently conjugate two proteins together. This kit is flexible so that researchers with little or no conjugation experience can make their own custom protein-protein conjugates to suit their needs. It includes all of the necessary components - including S-HyNic and S-4FB - and protocols for easy and specific crosslinking of any protein with any other protein of a different size greater than 7 kDa.
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TriLink® 2'-Deoxy-P-nucleoside-5'-Triphosphate
Supplier: TriLink BioTechnologies
2'-Deoxy-P-nucleoside is a degenerate base which behaves as a universal pyrimidine and therefore base pairs with both adenine and guanine. This feature makes it a powerful tool in mutagenesis studies.
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Digoxigenin kits, ChromaLink™ Digoxigenin One-Shot antibody labelling kit
Supplier: TriLink BioTechnologies
ChromaLink™ Didoxigenin contains a UV-traceable chromophore to enable reproducibility labelling. The degree of biotinylation can be measured in minutes, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple and direct UV scan, biotin incorporation can be quantified and reproducible production of consistent batches ensured. ChromaLink™ labelling can be paired with NanoLink™ Streptavidin Magnetic Beads for many types of assay development.
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TriLink® CleanCap® Renilla Luc mRNA (5 moU)
Supplier: TriLink BioTechnologies
Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.
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TriLink® 5-Propargylamino-2'-deoxycytidine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® 5-Propargylamino-2'-deoxycytidine-5'-Triphosphate
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TriLink® 2'-O-Methylcytidine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® 2'-O-Methylcytidine-5'-Triphosphate
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Conjugation reagents, linkers
Supplier: TriLink BioTechnologies
Protein assay, Conjugation reagent, SHNH Crosslinker (HyNic for technetium labelling)
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TriLink® 2'-Deoxythymidine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
TriLink® 2'-Deoxythymidine-5'-O-(1-Thiotriphosphate)
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TriLink® 2',3'-Dideoxyguanosine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
2',3'-Dideoxyguanosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddGTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.
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TriLink® 2',3'-Dideoxythymidine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
2',3'-Dideoxythymidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddTTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.
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TriLink® N6-Methyladenosine-5'-Triphosphate
Supplier: TriLink BioTechnologies
N6-methyl adenosine (N6-methyl ATP) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs (Meyer et al.). N6-methyl ATP can substitute for ATP in some biological systems, and is a potent agonist for P2Y-purinoceptors in the guinea pig, taenia coli (Burnstock et al.).
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TriLink® 7-Deaza-2'-deoxyguanosine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This deoxyguanosine analog lacks the N7 nitrogen found in the natural guanine nucleobase. Replacement of this nitrogen atom with a carbon atom results in diminished structure of the major groove in DNA and may disrupt base stacking, but does not interfere with Watson-Crick base pairing. This is desirable for applications such as amplification and sequencing of GC-rich nucleic acid sequences.
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TriLink® N7-Methyl-3'-O-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine
Supplier: TriLink BioTechnologies
TriLink® N7-Methyl-3'-O-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine
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Antibody labelling kits, R-PE-Antibody Conjugation kit
Supplier: TriLink BioTechnologies
The R-PE-Antibody Conjugation kit offers an innovative catalysed conjugation that delivers >95% conversion of antibody to conjugate. The kit includes everything needed to make two direct-labelled R-PE antibody conjugates from 500 µg of any user-supplied antibody.
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TriLink® CleanCap® M6 EPO mRNA (N1MePsU)
Supplier: TriLink BioTechnologies
EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA in vivo resulted in significant increases of both reticulocyte counts and hematocrits.