"Stemcell Technologies"

Supplier: Stemcell Technologies

Fetal bovine serum for cell culture and research applications.

Supplier: Stemcell Technologies

Defined, serum-free, xeno-free medium for pluripotent stem cells.

Supplier: Stemcell Technologies

Pre-aliquoted methylcellulose-based medium with recombinant cytokines (without EPO) for human cells. 24×3 ml vials.

Supplier: Stemcell Technologies

SARS-CoV-2 Recombinant Nucleocapsid Protein, aa1-419 is expressed in E. coli and is one of four structural proteins encoded by the SARS-CoV-2 genome. The Nucleocapsid Protein is transcribed from the viral “N” gene and is the protein that interacts with RNA to form the nucleocapsid. The protein is a homo-oligomer, and both the monomer and the oligomer can interact with RNA. This protein also interacts with the membrane protein (protein M) after infection of the host cell during packaging of the positive-strand viral genome RNA into the ribonucleocapsid during virion assembly. At the amino terminus, SARS-CoV-2 Recombinant Nucleocapsid Protein contains a thrombin site, a T7 tag, and a polyhistidine tag.

Supplier: Stemcell Technologies

Rat monoclonal IgM antibody against mouse CD49b (integrin α2), PE-conjugated.

Supplier: Stemcell Technologies

Defined, xeno-free induction medium for early mesodermal differentiation.

Supplier: Stemcell Technologies

Mouse monoclonal IgG1 antibody against human CD34, FITC-conjugated.

Supplier: Stemcell Technologies

For removing nucleases from DNA/RNA samples and terminating enzymatic reactions.

Supplier: Stemcell Technologies

For characterisation of human MSCs and their differentiation to chondrogenic, adipogenic, or osteogenic lineages.

Supplier: Stemcell Technologies

For digestion of native collagen fibrils.

Supplier: Stemcell Technologies

Density gradient medium for use with RosetteSep™ CD3+ T cell, CD4+ T cell, CD8+ T cell, and B cell enrichment cocktails.

Supplier: Stemcell Technologies

Interleukin 5 (IL-5) is a member of the short-chain 4-α-helical bundle subset of hematopoietic cytokines. It binds to a receptor consisting of IL-5Ra, which is specific for IL-5R, and common beta chain, which is shared with the receptor for IL-3 and GM-CSF (Shearer). Upon binding to its receptor, IL-5 activates the JAK/STAT and MAPK pathways. IL-5 is produced by Th2 cells, eosinophils, and activated mast cells. It functions in the recruitment, activation, proliferation, and survival of eosinophils, thus playing an important role in allergic inflammation, asthma, and parasite immunity. Stimulation of eosinophils with IL-5 leads to their activation, upregulation of CD11b expression, and inhibition of apoptosis (Shearer).

Supplier: Stemcell Technologies

Stimulating T cells with HIV-1 (B Gag) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HIV-1 (B Gag) Peptide Pool is a lyophilised mixture of 123 peptides from the gag polyprotein of human immunodeficiency virus 1 (HIV-1). The pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 500 on gag polyprotein. The HIV-1 gag polyprotein is a key mediator of viral particle assembly (Campbell and Rein; Dong et al.), and together with the endosomal sorting complexes required for transport (ESCRT), results in budding during virion release (Carlson et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: Stemcell Technologies

Thrombopoietin (TPO) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. TPO stimulates the proliferation and maturation of megakaryocytes and has an important role in regulating the level of circulating platelets in vivo (Bartley et al.; de Sauvage et al.; Foster et al.; Sohma et al.). TPO also promotes the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multilineage progenitor cells. It is commonly used with other cytokines such as stem cell factor (SCF) and Flt3/Flk-2 ligand to promote expansion of primitive hematopoietic cells in culture (Hitchcock and Kaushansky). The TPO receptor, c-Mpl, is expressed at all stages of megakaryopoiesis, from hematopoietic stem and progenitor cells to mature platelets (Ng et al.).

Supplier: Stemcell Technologies

Immunodensity depletion of CD45+ cells.

Supplier: Stemcell Technologies

Isolate or deplete highly purified HLA-DR+ cells from human PBMCs or leukapheresis samples by immunomagnetic selection.