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Ferene disodium salt, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt, also known as Ferene, is commonly used to measure the concentration of iron ions in aqueous solutions. The reagent is sensitive to ferrous iron ions (Fe2+) and forms a purple-colored complex with these ions in the presence of a reducing agent. The intensity of the purple color formed is proportional to the concentration of iron ions present in a solution and is quantified spectrophotometrically.

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4-(2-Pyridylazo)resorcinol 96%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

4-(2-Pyridylazo)resorcinol (PAR) is a monosodium salt(1), dibasic acid that forms the protonated complexes with most metal ions.

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Cesium chloride ≥98% Grade II, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

It is used for the preparation of electrically conducting glasses. Used to make solutions for the separation of RNA from DNA by density gradient centrifugation.

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Toluidine blue, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Toluidine blue (C.l. 52040) is a diaminothiazine dye. It is also known as TBO, tolonium chloride, and toluidine blue O.

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Rhodamine 110 ≥75% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Rhodamine 110 chloride (R110) is a laser grade dye. It has been used for the synthesis of the rhodamine 110 octadecyl ester (R110C18).

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3,3'-Diaminobenzidine tetrahydrochloride hydrate, ISOPAC®, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridisation. DAB (3,3′-Diaminobenzidine) is utilised in many applications for the visualisation of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidised DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.

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Alcian blue solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

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Methylene blue (Neisser) for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Methylene blue, Neisser formulation. Well developed granules of volutin (polyphosphate) may be seen in unstained wet preparations as round refractive bodies within the bacterial cytoplasm. With methylene blue they stain metachromatically, a reddish purple color.

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Direct blue 1, powder

Supplier: SIGMA ALDRICH MICROSCOPY

Chicago Sky Blue 6B is a counterstain for background autofluorescence in fluorescence and immunofluorescence histochemistry. It has also been used as a filling in glass pipette electrodes for electrophysiological recordings in the brain sections of rats.

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10-(2′,4′-Dinitrophenylazo)-9-phenanthrol, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

10-(2',4'-Dinitrophenylazo)-9-phenanthrol, (DNAF)

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APC-Cy5.5 Tandem 4 mg/ml, suspension, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Phycobiliprotein exhibiting exceptional absorbance and fluorescence characteristics due to a lack of susceptibility to internal and external fluorescence quenching. Ideal for highly sensitive studies in flow cytometry and immunoassays.

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Bromocresol green-methyl red solution mixed indicator, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Suitable for titration of morpholine

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Brilliant green ≥90% (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Brilliant green ≥90% (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®

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Coomassie® brilliant blue R-250 ~50% (dye content), Technical Grade, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Brilliant Blue R (C.l. 42660) is a triaminotriarylmethane dye with two amino groups being benzylated and one phenylated. It is also known as brilliant indocyanine 6B and coomassie blue R250. Brilliant Blue R has been widely used for protein band detection after SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).

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Fast green FCF, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fast green FCF, Sigma-Aldrich®

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Trypan blue 60% (dye content) ≥80% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B. Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

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Proflavine hemisulphate hydrate, powder, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Proflavine is a small, amphipathic, acridine-derived fluorescent dye.Proflavine hemisulfate is used as a rapid stain for cytological studies of biological specimens. It stains cytoplasmic structures and nuclei. Proflavine has the capability to intercalate double stranded DNA.

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Anthrone in ACS Reagent grade water 97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Anthrone, also known as 9(10H)-Anthracenone, is a planar tricyclic aromatic ketone. In acidic conditions, anthrone reacts with carbohydrate to yield a blue-green colour.

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2,7,12,17-Tetra-tert-butyl-5,10,15,20-tetraaza-21H,23H-porphine, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

2,7,12,17-Tetra-tert-butyl-5,10,15,20-tetraaza-21H,23H-porphine has been used in multifunctional drug delivery investigations from ionic triblock terpolymer micelles.

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Osteo-Bed Bone Embedding Solvent

Supplier: SIGMA ALDRICH MICROSCOPY

For use with Osteo-bed bone embedding kit. Solvent for removal of plastic from sections prior to rehydration and staining. Most histochemical and immunohistochemistry staining protocols require the removal of methyl methacrylate (MMA) and rehydration of mounted sections for improved stain penetration and greater contrast.

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Nigrosine, certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Nigrosin,certified by the Biological Stain Commission (Acid black 2; Nigrosin water soluble).

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Thioflavin T stain for amyloid, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Thioflavin T is a benzothiazole dye that increases in fluorescence upon binding to amyloid. Thioflavin T has been used in histology and for protein characterization.

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Acetate solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for use in the Leukocyte Acid Phosphatase procedure, procedure No. 386 or 387

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Crystal violet lactone 97% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Crystal Violet lactone, Dye content 97%.

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Carbazole ≥95% (by GC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Carbazole is an aromatic heterocyclic organic molecule.

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Violamine R 60% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Violamine R 60% (dye content), Sigma-Aldrich®

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Oil Red EGN, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Oil Red EGN has been used to stain lipid droplets accumulated in cell during adipogenesis.

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Silver Stain (Modified GMS) Kit, Sigma-Aldrich®

Silver Stain (Modified GMS) Kit, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Silver stain is an aid in distinguishing fungi, basement membrane and opportunistic organisms such as Pneumocystis carinii in tissue sections or smears.

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4-(Dimethylamino)azobenzene-4'-isothiocyanate 97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

4-(4-Isothiocyanatophenylazo)-N,N-dimethylaniline,97% (4-(Dimethylamino)azobenzene-4'-isothiocyanate; DABITC)

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Astrazon Orange G, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Metachromatic dye

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