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Acridine orange base ≥75% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye. Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.

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FURA-2 pentapotassium salt, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Cell impermeable fluorescent probe for Ca2+.

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Wright's stain modified, Sigma-Aldrich®

Wright's stain modified, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Popular hematology stain used for differentially staining the cellular elements of blood. For dip, rack, and batch staining techniques.

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Sudan Black B Staining System, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for the histochemical demonstration of neutrophil granules in blood or bone marrow films.

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2-Phenylaniline 97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

2-Aminobiphenyl is a substrate for UGTs (UDP-glucuronosyltransferases), including UGT1A4, UGT2B13 and UGT2B16.

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5-Aminofluorescein (isomer I), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoresceinamine, isomer I has been used as a probe in immobilization and is used to determine its ability to potentiate the antiviral activity of poly r (A-U).

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Dihydroethidium ≥95%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Redox indicator. Blue fluorescence until oxidized to ethidium. Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC).

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3-Indolyl-ß-D-galactopyranoside, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

3-Indolyl-ß-D-galactopyranoside, Sigma-Aldrich®

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1-Methoxy-5-methylphenazinium methyl sulphate ≥95%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

1-Methoxy-5-methylphenazinium methyl sulfate (MPMS) is a photochemically stable electron-transport mediator between NAD(P)H and tetrazolium dyes. The reduced form of MPMS converts tetrazolium salt to purple precipitate formazan. It is a very stable and non-enzymic electron carrier that mediates electron transfer by dehydrogenases in vitro to various electron acceptors such as tetrazolium dyes or the electrode of an enzymic electric cell.

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Erythrosin B disodium salt, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Erythrosin B disodium salt, Sigma-Aldrich®

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Acid Phosphatase, Leukocyte (TRAP) Kit Kit formulated with all liquid reagents, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Kit is used to demonstrate acid phosphatase and tartrate resistant acid phosphatase (TRAP) in blood, bone marrow and tissue touch preparations.

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Sodium diatrizoate hydrate ≥98%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Sodium diatrizoate hydrate has been used in spore purification. It has also been used in the isolation of plasma lymphocytes, monocytes, and dendritic cells. Sodium diatrizoate is also suitable as a density gradient reagent in blood cell separation. It has also been utilised as a radiological contrast agent.

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Differentiation Solution

Supplier: SIGMA ALDRICH MICROSCOPY

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

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FITC (fluorescein-5-isothiocyanate, fluorescein isothiocyanate isomer I) ≥97.5% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins. Fluorescein isothiocyanate isomer I has been used for the staining of conidia.
Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC).

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Chrysophenine 65% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Chrysophenine, Dye content 65 % (Direct Yellow 12)

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5-(Iodoacetamido)fluorescein ≥90% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

5-(Iodoacetamido)fluorescein. ≥90% (HPLC) (5-IAF)

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Fast black K salt, Practical grade, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fast black K (C.l. 37190) is a lipophilic amino-substituted monoazo dye. It is also known as Brentamine fast black K salt.

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Rosolic acid (Aurin) 84% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

p-Rosolic acid (C.I. 43800) is an acid-baseindicator from the triphenylmethane group. It is also known as aurin and 4-[Bis(4-hydroxyphenyl)methylene]-2,5-cyclohexadienone.

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4-(Dimethylamino)benzaldehyde, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Suitable for histochemical demonstration of nitro blue tetrazolium reduction in neutrophils.

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Nitro blue tetrazolium chloride (NBT), tablets, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Nitro Blue Tetrazolium is a tetrazole compound with two tetrazole rings.

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Eosin B, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Eosin B, Sigma-Aldrich®

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Fast Red Violet LB Base solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Used as a kit component in Procedure 91 for the demonstration of specific esterase activity in peripheral blood smears or paraffin sections.

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2',7'-Dichlorofluorescein ~90% (by TLC), powder chemical indicator, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species). 2′,7′-Dichlorofluorescein has been used as an oxidation-sensitive fluorescent probe to measure ROS (reactive oxygen species) formation in cells.

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Resazurin sodium salt, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Resazurin sodium salt, Sigma-Aldrich®

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Orcein, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Orcein, Sigma-Aldrich®

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Aluminon ACS, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Reagent for aluminium. Aurintricarboxylic acid readily polymerises in aqueous solution, forming a stable free radical that inhibits protein-nucleic acid interactions. It is a potent inhibitor of ribonuclease and topoisomerase II by preventing the binding of the nucleic acid to the enzyme. It stimulates tyrosine phosphorylation processes including the Jak2/STAT5 pathway in NB2 lymphoma cells, ErbB4 in neuroblastoma cells, and MAP kinases, Shc proteins, phosphatidylinositide 3-kinase and phospholipase C in PC12 cells. Aurintricarboxylic acid inhibits apoptosis in many cell types. Its neuroprotective effect, perhaps due to its ability to prevent down-regulation of Ca²⁺ impermeable GluR2 receptors or to its ability to inhibit calpain, a Ca²⁺-activated protease that is activated during apoptosis.

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3,5-Dichloro-2-hydroxybenzenesulphonic acid sodium salt suitable for peroxidase analysis, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Sodium 3,5-dichloro-2-hydroxybenzenesulfonate is used in conjunction with 4-aminoantipyrine (4-AAP) and hydrogen peroxide (H2O2) for chromogenic quantitation of uric acid in serum and urine samples.

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Paraplast Plus®, pellets for electron microscopy (fast-melting), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Regular Paraplast with approx. 0,8% DMSO added for enhanced ease of infiltration and sectioning. Paraffin polyisobutylene mixture is a tissue embedding medium composed of highly purified paraffin and polyisobutylene and a low level of dimethyl sulfoxide (DMSO) to enhance the rate of tissue penetration. Paraffin-polyisobutylene (76258) supports thin wrinkle-free sectioning down to 4 µm with more flexible and continuous ribbons.

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Rhodamine 19 perchlorate ~95% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Rhodamine 19 perchlorate ~95% (dye content), Sigma-Aldrich®

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Collodion 2% in amyl acetate for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Solution 2% in amyl acetate (Cellulose nitrate).

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