Supplier: SIGMA ALDRICH MICROSCOPY

N-Benzylidenebenzenesulfonamide,97%

Supplier: SIGMA ALDRICH MICROSCOPY

ACCUSPIN System-Histopaque-1077 products are intended for use in the isolation of lymphocytes and other mononuclear cells. The separation medium, Histopaque-1077, is a sterile-filtered, endotoxin-tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1,077 g/ml. ACCUSPIN System-HISTOPAQUE-1077 employs γ-irradiated 12mL polypropylene centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ('frit'). Each tube is aseptically filled with 3 mL of Histopaque 1077. Each tube will separate 3 to 6 ml of anticoagulated blood.

Supplier: SIGMA ALDRICH MICROSCOPY

Aurintricarboxylic acid ammonium salt has been used as a nuclease inhibitor to study its effects on transfection.

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Kit component for Sigma Alkaline Phosphatase Procedure, kits 86C and 86R

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May be used as a substitute for trypan blue for some biological and staining applications.

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Haematoxylin

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Silane-prep slides are microscope slides coated with silane (aminoalkysilane) to improve the adhesion of tissues to the slide for immunohistochemistry. The slide includes a 'frosted end' for slide/patient identification. It is not tested for use in microarray assays.

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Kit component in the silver stain kit.

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Tetrabromophenol Blue sodium salt, Dye content 85% (3,3',3'',4,5,5',5'',6-Octabromophenolsulfonphthalein sodium salt).

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Intended for use with Carbol Fuchsin (HT80-1) in Sigma Acid Fast Stain, Procedure HT80. Acts as both a counterstain and a differentiator.

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Ponceau S solution has been used for membrane staining (Western blotting).

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4-Phenoxyphthalonitrile,98%

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4-Formylbenzene-1,3-disulfonic acid disodium salt hydrate,97% (Benzaldehyde 2,4-disulfonic acid disodium salt; Sodium 4-formylbenzene-1,3-disulfonate).

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Intended for use in the Silver Stain (Steiner-Steiner) kit. Hydroquinone, 0.5 g each, with excipients.

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4-Nitroguaiacol potassium salt hydrate,98% (2-Methoxy-4-nitrophenol potassium salt)

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Azure B eosinate, Sigma-Aldrich®

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An intracellular fluorescent pH indicator.

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Standard procedure and microwave procedure included in package insert.
When treated with periodic acid, glycols are oxidized to aldehydes. After reaction with Schiff′s Reagent (a mixture of pararosaniline and sodium metabisulfite), a pararosaniline adduct

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Metanil yellow has been used for staining of leukocyte granules. It is also used as a counterstain in microscopic sections.

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Pinacyanol chloride, (1,1'-Diethyl-2,2'-carbocyanine chloride; 2,2'-Trimethinequinocyanine chloride; Quinaldine blue).

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A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density.

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Fast Garnet GBC base has been used as a stain in papain activity inhibition assay, to stain for tryptase-activity and chymase-like activity, to gel stain for esterolytic activity.

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Pyrocatechol Violet (PV) is a sulfone phthalein dye. It chelates metal ions. The metal-PV complex is bluish-violet in colour.

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4-tert-Butylphthalic anhydride has been used to synthesise novel yellow quinophthalone dyes for LCD colour filters.

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Inhibits mitochondrial Ca2+ uniporter; capsaicin antagonist; blocks VR1 vanilloid receptor-coupled ion channels.

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Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.

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Ethidium homodimer is a red fluorescent dye. It cannot cross an intact plasma membrane, but can easily pass through a damaged plasma membrane. Ethidium homodimer is used to check membrane integrity. One molecule of EthD-1 will non-selectively bind four base pairs in dsDNA.

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Nuclear Fast Red is suitable for the determination of calcium. It is suitable for use in prussian blue staining in neural progenitor cells. It may be employed as highly sensitive 'off/on' fluorescent probe for the determination of guanine. Nuclear fast red (2-anthracenesulfonic acid, 4-amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-, monosodium salt) may be used as selective complexing agent for the simultaneous determination of copper and bismuth by adsorptive stripping voltammetry.

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Indicator for organolithium assay.

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2-(Ethylthio)benzothiazole,97%