73790 Results for: "MP Biomedicals"

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5-Bromo-2’-deoxyuridine is a thymidine analog and useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. It can be used to label DNA which can be used in conjunction with BrdU Monoclonal Antibody for the immunohistochemical analysis of cell proliferation.

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5-Bromo-2'-deoxycytidine can be incorporated into DNA in the presence of DNA polymerase, replacing dCTP.

Supplier: MP Biomedicals

Dithranol (1, 8 - dihydroxy-9-anthrone) is also known as anthralin. It is a vital topical antipsoriatic drugs. Dithranol, an athracene derivative, is a free radical generating mitochondrial poison that induces apoptosis in keratinocytes.

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N-Methylglucamine is used in the synthesis of surface active agents, pharmaceuticals, dyes; an antimonate as antiprotozoal. Used as a Na⁺ substitute in systems to maintain physiological solutions as isotonic but without sodium. Has also been used in the studies of rabbit cerebral artery, rabbit cardiac myocytes, perfused rat intestine, and frog skeletal muscle.

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Dithiothreitol (DTT, Cleland's reagent) ≥99% for molecular biology

Supplier: MP Biomedicals

The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized

The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/mL using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected.

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RPMI is a general purpose media with a broad range of applications for mammalian cells.

Supplier: MP Biomedicals

Cellobiose is a disaccharide composed of two glucose molecules. These are β-1,4-glycosidically linked. It is reducing sugar, which is readily soluble in polar solvents such as water and ethanol. D-Cellobiose is a substrate of β-glucosidase.

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Cetyl alcohol is used in the cosmetic industry as an opacifier in shampoos, or as an emollient, emulsifier or thickening agent in the manufacture of skin creams and lotions. It is also employed as a lubricant for nuts and bolts.

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Sorbic acid (2,4-hexadienoic acid) is a straight chain unsaturated fatty acid. It is an antimicrobial agent. The antimicrobial action of sorbic acid is due to its inhibitory influence on various enzymes in the microbial cell such as enzymes involved in carbohydrate metabolism such as enolase and lactate dehydrogenase, enzymes of citric acid cycles.

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Rabbit Anti -Horse serum was developed in rabbit.

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Anti-Goat Serum Rabbit Polyclonal Antibody

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Based on Immunoelectrophoresis, it produces precipitin band at a concentration of 1,0 mg/ml.

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Ammonium persulphate is a strong oxidising agent. It is a radical initiator.

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Methylene blue has been used as a stain in bacteriology and as an oxidation-reduction indicator. A sensitive stain for RNA and
ribonuclease. An ethidium bromide alternative for the visualization of nucleic acids in gels:

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CHAPS is useful for solubilising membrane proteins. Frequently used prior to electrophoresis since it generally does not denature the proteins.

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Soluble in water (357 mg/ml at 25 °C; 384 mg/ml at 100 °C), glycerol (100 mg/ml); very slightly soluble in ethanol. Solubility in water is decreased by HCl; almost insoluble in concentrated HCl. Aqueous solutions has a neutral pH (6,7 to 7,3).

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Potassium sulfate is used in various industrial and research applications. Industrial uses include fertilizer production, the manufacture of potassium alum, potassium carbonate, and glass.

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Potassium ferricyanide is the chemical compound with the formula K3[Fe(CN)6]. This bright red salt contains the octahedrally coordinated [Fe(CN)6]3- ion. It is soluble in water and its solution shows some green-yellow fluorescence.

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A high viscosity mucopolysaccharide composed of repeating dimeric units of glucuronic acid and N-acetyl glucosamine which forms the core of complex proteoglycan aggregates found in extracellular matrix.

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Aldolase is a tetrameric protein. It catalyses a key reaction in glycolysis and energy production:D-Fructose 1,6-bisphosphate aldolase → dihydroxyacetone phosphate + D-glyceraldehyde-3-phosphate. Aldolase is present in all animal tissue and in most microorganisms. There are two classes of aldolases. Class I aldolase is found in animal and higher plant tissue. Class II aldolase is found in primitrive cells such as yeasts and bacteria. Class I aldolase is characterised by not requiring a bivalent metal cofactor and the formation of a ketimine Schiff base intermediate with the substrate dihydroxyacetone phosphate. Class II aldolase requires a metal cofactor and is inhibited by EDTA. Three types of aldolase exist in animal tissue. The major form, type A is found in muscle; type B is found in liver tissue and type C (plus some type A) is found in brain tissue. Aldolase forms five isozymes which may to various degrees be organ specific.

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Soluble in water.

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Sodium borate decahydrate is a mineral, and a salt of boric acid. The term borax is used for a number of closely related minerals or chemical compounds that differ in their crystal water content, but usually refers to the decahydrate.

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Sodium phosphate monobasic monohydrate is useful in conjunction with Sodium Phosphate, Dibasic in the preparation of biological buffers. Used in many applications including the purification of antibodies.

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This antibody localises specifically the neurofilament using indirect immunofluorescent labeling on formalin fixed paraffin embedded tissue sections and the immunoblot technique. Good immunofluorescent labeling may be obtained in human, porcine, rat, chicken, guinea pig, rabbit and mouse tissue.

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(S)-(+)-Camptothecin binds irreversibly to the DNA-topoisomerase I complex, inhibiting the reassociation of DNA after cleavage by topoisomerase I and traps the enzyme in a covalent linkage with DNA.

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Esculin Monohydrate protects cells against DNA damage and reduces ROS levels. It is also a carcinogenesis inhibitor and displays vitamin P activity.

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Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic, and tribasic (K3PO4). Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.

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Potassium phosphate is typically used as a component for a wide variety of media used in the culture of microorganisms, as a component in phosphate buffered saline (PBS). In addition to helping maintain pH, it supplies essential phosphate.

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This powdered salt mixture is used as animal diet.