Supplier: STEMCELL Technologies

Human (Actin) peptide pool is a lyophilised mixture of 92 peptides from human actin, alpha skeletal muscle, and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 377 on actin. It is frequently used as a negative control. Actin, alpha skeletal muscle, is part of the highly conserved actin protein family and is the predominant isoform found in adult skeletal muscle (Laing et al.). Peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: VWR Collection

Advanced routine fluorescence microscope for transmitted brightfield and fluorescence observations with high quality IOS W-Plan or IOS W-Plan F (Semi-Apo) objectives. Suitable for educational and laboratory applications.

Supplier: THERMO SAVANT

Thermo Scientific™ Savant™ SpeedVac™ SPD300P1-230 vacuum concentrator is a large-capacity vacuum concentrator with a modular design, ideal for drying aggressive organic solvents, strong acids, bases, and combinatorial chemistry solvents. It is capable of drying larger quantities of samples at one time and can support high-volume sample preparation needs.

Supplier: Biotium

PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive dye that binds to dsDNA with high affinity. Upon photolysis with visible light, PMA dye covalently attaches itself to dsDNA.

Supplier: STEMCELL Technologies

Stimulating T cells with Candida (MP65) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. Candida peptide pool is a lyophilised mixture of 92 peptides from the mannoprotein MP65 of Candida albicans (yeast), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 379 on MP65. MP65 contributes to cell wall integrity and epithelia adherence (Sandini et al.), and is a potent T cell immunogen (Gomez et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with EBV (HLA Class I Control) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (HLA Class I Control) Peptide Pool is a lyophilised mixture of 26 peptides from Epstein-Barr virus (EBV), and consists of defined HLA class I-restricted T cell epitopes. Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development. For consistency and reproducibility across your applications, CMV peptide pool from STEMCELL has a purity ≥95%.

Supplier: STEMCELL Technologies

Stimulating T cells with the HPV18 (L1) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. A lyophilised mixture of 140 peptides from major capsid protein L1 of human papillomavirus type 18 (HPV18), HPV18 (L1) Peptide pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 568 on L1. L1 enters the nuclei of host cells and can assemble into virus-like particles (Stauffer et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with HHV8 (K8.1) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HHV8 (K8.1) Peptide pool is a lyophilised mixture of 55 peptides from glycoprotein K8.1 of human herpesvirus 8 (HHV-8) and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 228 on K8.1. Expressed in the late-lytic phase, K8.1 is a viral envelope protein that binds to heparin sulfate on the cell surface and facilitates HHV 8 infection (Zoeteweij et al; Birkmann et al.; Wang et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with SARS-CoV-2 (Spike Protein) Delta/B.1.617.2 Mutation Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. This peptide pool is a lyophilised mixture of 27 peptides from the spike glycoprotein of the SARS-CoV-2 Delta variant (B.1.617.2) that selectively cover the mutated regions in the spike protein. Through interactions with the angiotensin-converting enzyme 2 (ACE2) receptor, the spike protein enables the virus to attach to the cell membrane, and plays a critical role in viral entry (Hoffmann et al.; Walls et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with HIV-1 (B Gag) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HIV-1 (B Gag) Peptide Pool is a lyophilised mixture of 123 peptides from the gag polyprotein of human immunodeficiency virus 1 (HIV-1). The pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 500 on gag polyprotein. The HIV-1 gag polyprotein is a key mediator of viral particle assembly (Campbell and Rein; Dong et al.), and together with the endosomal sorting complexes required for transport (ESCRT), results in budding during virion release (Carlson et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with Clostridium (Tetanus Toxin) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. Clostridium Peptide pool is a lyophilised mixture of 326 peptides from tetanus toxin of Clostridium tetani (strain Massachusetts/E88), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 1315 on tetanus toxin. Tetanus toxin entry into central neurons is mediated by the synaptic vesicle-binding protein SV2 (Yeh et al.), where it selectively cleaves synaptobrevin (Link et al.; Schiavo et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with EBV (LMP2A) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (LMP2A) peptide pool is a lyophilised mixture of 27 peptides from latent membrane protein 2A (LMP2A) of Epstein-Barr virus (EBV; strain GD1), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 118 on LMP2A. LMP2A contributes to the survival of EBV-transformed B cells (Mancao and Hammerschmidt) and mediates partial escape of infected B cells from recognition by EBV-specific CD8+ T cells (Rancan et al). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: Thermo Fisher Scientific

The Thermo Scientific KingFisher Apex represents the pinnacle of automated purification for DNA, RNA, proteins, and cells. Building on decades of KingFisher product value and excellence, we’ve combined superior instrument capabilities with complete touch screen-based control to deliver unrivalled flexibility and performance. The KingFisher Apex makes sample prep simple.

Supplier: STEMCELL Technologies

Stimulating T cells with HHV1 (gD) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HHV1 (gD) Peptide pool is a lyophilised mixture of 96 peptides from envelope glycoprotein D of human herpesvirus 1 (HHV1; strain Patton), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 394 on gD. gD is required at the first stage of virus-induced membrane fusion (Subramanian and Geraghty), which likely occurs via binding to receptors such as nectin-1 (Giovine et al.) and HVEM (Connolly et al.) on cell surfaces. Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with EBV (LMP1) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (LMP1) Peptide pool is a lyophilised mixture of 94 peptides from latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV; strain B95-8), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 386 on LMP1. LMP1 is essential for EBV-mediated growth transformation of B cells (Kaye et al.) and has been shown to downregulate toll-like receptor 9 (TLR9) expression in infected cells, a mechanism the virus may use to escape host innate immune recognition (Fathallah et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with SARS-CoV-2 (Spike Protein) Omicron/B.1.1.529 Mutation Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. A lyophilised mixture of 80 peptides of the spike glycoprotein of SARS-CoV-2 Omicron variant (B.1.1.529), these peptides selectively cover the mutated regions in the spike protein of the SARS-CoV-2 Omicron variant, compared to wild-type. Through interactions with the angiotensin-converting enzyme 2 (ACE2) receptor, the spike protein enables the virus to attach to the cell membrane, and plays a critical role in viral entry (Hoffmann et al.; Walls et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with JCV (LT) Peptide Pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. This peptide pool is a lyophilised mixture of 170 peptides from large T antigen (LT) of JC polyomavirus (JCV), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 688 on LT. LT is detectable during JCV latency (Rossi et al.), and in immunocompromised patients with JVC-associated pathologies such as progressive multifocal leukoencephalopathy (PML) (Delbue et al.). In PML, LT has been associated with activating the promoter of survivin, an anti-apoptotic protein (Del Valle et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with HPV16 (E7) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HPV16 (E7) Peptide pool is a lyophilised mixture of 22 peptides from protein E7 of human papillomavirus type 16 (HPV16) consisting of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 98 on protein E7. E7 is an oncoprotein that binds and mediates degradation of the retinoblastoma tumor suppressor protein pRB (Gonzalez et al.). E7 together with E6 demonstrated the ability to immortalize human keratinocytes in vitro, which likely has significance for HPVs oncogenic potential (Hawley-Nelson et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with HPV18 (L2) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. A lyophilised mixture of 113 peptides from minor capsid protein L2 of human papillomavirus type 18 (HPV18), HPV18 (L2) peptide pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 462 on L2. With two nuclear localization signals, L2 may enter the nuclei of host cells via several import pathways mediated by Kap α2β1 heterodimers, Kap β2, and Kap β (Klucevsek et al.). L2 can also assemble into virus-like particles, and may have a role in encapsidation (Stauffer et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with HHV6 (U54) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HHV6 (U54) peptide pool is a lyophilised mixture of 112 peptides from protein U54 of human herpesvirus 6B (HHV-6B; strain Z29), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 459 on U54. U54 is a tegument transactivator and is suggested to be a homolog of the CMV UL82/83 (pp65/72K) protein (Isegawa et al.; Nicholas). In infected T cells, U54 has been shown to inhibit gene transcription of interleukin 2 (IL-2), the T cell growth factor, which likely contributes to viral persistence (Iampietro et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with EBV (GP350/340) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (GP350/340) Peptide pool is a lyophilised mixture of 224 peptides from envelope glycoprotein GP350 (GP350/340) of Epstein-Barr virus (EBV; strain B95-8), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 907 on GP350/340. GP350 meditates EBV attachment to B lymphocytes via binding to the cell surface receptor CR2 (also known as C3d receptor or CD21) (Nemerow et al.; Tanner et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with EBV (EBNA-3B) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (EBNA-3B) Peptide Pool is a lyophilised mixture of 234 peptides from Epstein-Barr nuclear antigen 3B (EBNA-3B) of Epstein-Barr virus (EBV; strain AG876) and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 946 on EBNA-3B. EBNA-3B is considered non-essential for B cell growth transformation (Chen et al., 2005; Tomkinson and Kieff), but has been shown to regulate the expression of cellular genes in EBV-infected cells (Chen at al., 2006). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with JCV (VP1) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. This peptide pool is a lyophilised mixture of 86 peptides from major capsid protein VP1 (VP1) of the JC polyomavirus (JCV), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 354 on VP1. VP1 binds LSTc oligosaccharides found on host cell glycoproteins and glycolipids, and this interaction is said to be essential for JCV viral growth and infectivity (Neu et al.). The JCV VP1 (Del Valle et al.) and large T antigen (LT) (Delbue et al.) are detected in JCV-associated pathologies such as progressive multifocal leukoencephalopathy (PML) in patients with HIV. Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with CMV (IE-2) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. CMV (IE-2) Peptide pool is a lyophilised mixture of 143 peptides from immediate early protein 2 (IE2) of human cytomegalovirus (CMV; strain AD169), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 580 on IE2. IE2 is critical for viral infection and for the expression of CMV early genes (Marchini et al.). It also controls transcription initiation for a subset of early-late and late viral promoters via RNA polymerase II (Li et al.). IE2, along with IE1, may contribute to the survival of CMV-infected cells by blocking tumor necrosis factor alpha (TNF-α)-induced apoptosis (Zhu et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with HPV16 (L2) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. A lyophilised mixture of 116 peptides from minor capsid protein L2 of human papillomavirus type 16 (HPV16), HPV16 (L2) Peptide Pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 473 on L2. L2 is equipped with multiple nuclear retention signals (Darshan et al.; Mamoor et al.), and enters the nuclei of host cells via several import pathways mediated by Kapα2β1 heterodimers, Kapβ2, and Kapβ3 (Darshan et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with BKV (LT) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. BKV Peptide pool is a lyophilised mixture of 171 peptides from the Large T (LT) antigen of BK polyomavirus (BKV), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 695 on LT antigen. LT antigen is an early viral protein (Low et al.) that contributes to the transformation and maintenance of host cells (Nakshatri et al.). It has been found to bind to a subset of tumor suppressor proteins (Harris et al.), and its co-expression with p53 has been observed in polyomavirus-associated nephropathy (Seemayer et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with HHV8 (K8) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HHV8 (K8) peptide pool is a lyophilised mixture of 57 peptides from protein K8 alpha of human herpesvirus 8 (HHV-8), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 237 on K8. A basic leucine zipper (bZip) protein that associates with the lytic origin of HHV8 (Lin et al.), K8 is thought to play a role in lytic DNA replication, as a trans-acting factor (AuCoin et al.). It has also been found to modulate the activity of the transcriptional transactivator K-Rta (Izumiya et al.), and has an autoregulatory function (Wang et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with VZV (IE63) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. VZV (IE63) peptide pool is a lyophilised mixture of 67 peptides from the transcriptional regulator ICP22 homolog (IE63) of Varicella-zoster virus (VZV; strain Oka vaccine), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 278 on IE63. Expressed during VZV latency and reactivation (Lungu et al.), IE63 demonstrates repressive activity in both the cytoplasm and nucleus of infected cells (Bontems et al.). Host interferon alpha (IFN-α) activity is inhibited by VZV IE63, which likely contributes to VZV pathogenesis (Ambagala and Cohen). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with CMV (UL44) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. CMV (UL44) peptide pool is a lyophilised mixture of 106 peptides from DNA polymerase processivity factor UL44 of human cytomegalovirus (CMV; strain AD169), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 433 on UL44. UL44 contains a nuclear localization signal (Alvisi et al.) and may bind DNA directly, or form a C clamp partially surrounding it, similar to proliferation cell nuclear antigen (PCNA) (Appleton et al.). UL44 has also been found to interact with viral (Kim and Ahn) and host (Strang et al.) proteins that contribute to viral DNA replication and efficient viral growth. Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with EBV (EBNA-3A) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (EBNA-3A) Peptide pool is a lyophilised mixture of 234 peptides from Epstein-Barr nuclear antigen 3 (EBNA-3A) of Epstein-Barr virus (EBV; strain B95-8) and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 944 on EBNA-3A. EBNA-3A contributes to B cell growth transformation (Hertle et al.; Tomkinson et al.) and has been found to interact with host cell proteins, where it activates (Young et al.) or represses (Cludts and Farrell; Hickabottom et al.) transcription of specific cellular genes. Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.