Cation exchange chromatography columns, HiTrap SP HP
Product Details & Documents
HiTrap SP HP is a strong cation exchange chromatography column for high-resolution, small-scale protein purification.
- Packed with SP Sepharose High Performance strong cation exchange resin
- Small (34 µm) bead size delivers high-performance, high-resolution purifications
- Can be used alone or connected in series
- Convenient HiTrap format: designed for use with a syringe, peristaltic pump, or chromatography system such as an ÄKTA system
With a bead size of 34 µm, SP Sepharose HP resin provides good resolution at a broad pH range, allowing selection of the most suitable pH for each application.
These columns packed with SP Sepharose High Performance resin are utilized in a variety of applications, including:
• Purification of cell extract during rapid purification of recombinant histones
• Purification of BpsOMP38 to research porin involvement in cephalosporin and carbapenem resistance of Burkholderia pseudomallei
• Use with an ÄKTA FPLC system forexpansion of hematopoietic stem cells by recombinant hoxb4
Specifications
- Flow rate:4 ml/min
- Particle Size:34 µm
- Capacity:1 ml
- Description:HiTrap™ SP HP
- Seq:02
- Exclusion limit:4×10⁶ (Mr) (globular proteins)
- Column hardware:PP
- Bed dimensions:7×25 mm
- Cat. No.:29-0513-24
- Max. pressure:5 bar
- Storage:4 to 30 °C, 20% Ethanol, 0,2 M Sodium Acetate
- pH range:4 - 13
#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 31770.294
Supplier: VWR Chemicals
#: 83645.320
Supplier: VWR Chemicals
#: 1.09439.1000
Supplier: Merck