The Pierce™ Coomassie Plus Protein assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.

• Colorimetric measure with a standard spectrophotometer or plate reader (595 nm)
• Easy to use single reagent; no working reagent preparation required
• Fast almost immediate colour development; add, mix and read results
• Broad range detects protein concentration in the range 1 to 1500 µg/ml
• Better improved linearity and response-uniformity compared to traditional Bradford formulations
• Flexible microplate and cuvette protocols provided with the instructions and adaptable to several target working ranges

This modification of the well-known Bradford method greatly reduces the tendency of Coomassie reagents to give non linear response curves by a formulation that substantially improves linearity for a defined range of protein concentration. In addition, the Coomassie Plus Reagent results in significantly less protein-to-protein variation than is observed with other Bradford-type Coomassie formulations.