Affinity chromatography media, Glutathione Sepharose™ 4 Fast Flow
: Cytiva
Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.
- For affinity chromatography
- Fast, one-step purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- For scale-up due to high flow properties
- Glutathione coupled to Sepharose 4 Fast Flow via a 10-carbon spacer arm
- Epoxy activation coupling method
- Stable in all commonly used aqueous buffers
Mild elution conditions preserve protein antigenicity and function. Sepharose 4 Fast Flow provides good flow properties and is excellent for scaling up. Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
- pH range:3 - 12
- Matrix:Highly cross-linked 4% agarose
- Ligand:Glutathione and 10-carbon linker arm
- Storage temperature:4 - 30 °C
- Binding capacity:>10 mg/ml
- Flow rate:<450 cm/h
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