Transformation of E. coli with pGAL™
EDVO221
: EDVOTEK
- Description:Transformation of E. coli with pGAL™
- Environmentally Preferable:
- Pk:1
In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the lacZ gene. IPTG is not required in this experiment since pGAL contains the complete lacZ gene.
- For 10 lab groups
- Complete in 50 minutes and grow overnight
Also required: 37 °C incubator, water bath.
: Kit includes instructions, LyphoCells, plasmid DNA, buffer, media, ampicillin, X-Gal, ReadyPour agar, petri dishes, sterile pipettes, loops and microtubes.