Simple, rapid extraction (no purification) of PCR-ready DNA from challenging FFPE tissue samples.

• Fast: PCR-ready DNA in minutes, not days
• Simple: No spin columns or transfer steps are required
• Compatible: Extracted DNA is compatible with both real-time and endpoint PCR
• Safe: No xylene or phenol extractions used

The analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the extensive cross-linking of all tissue components during the fixation process.

The QuickExtract™ FFPE DNA Extraction Kit is a fast, simple, and inexpensive method for preparing genomic DNA for PCR amplification from archival samples. The protocol requires only heat treatment to melt the paraffin, lyse the cells, decrease the fomalin-induced cross-linking in the sample, and degrade compounds inhibitory to amplification. Following heat treatment, the DNA sample is ready for PCR.

These challenges include chemical modification of the DNA, cross-linking of DNA with other molecules, degradation of the DNA, and the limited amount of nucleic acid in the samples.