Sepharose Chromatography Resins, Strep-Tactin® XT

A chromatography resin for purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag. These proteins bind very specifically to the immobilised Strep-Tactin XT ligand giving highly pure target protein.

Highly pure Strep-tag II and Twin-Strep-tag recombinant proteins
Fast purifications with simple elution
Physiological conditions and mild elution preserve target protein activity
Fast and easy regeneration
Compatible with a wide range of detergents, denaturants, and other additives

Strep-Tactin XT Sepharose contains the latest Strep-Tactin ligand, XT, which provides higher affinity, resulting in higher yields and lower loss of valuable target protein than with the predecessor product, StrepTactin sepharose high performance (HP). Strep-Tactin XT Sepharose also enables purification under denaturing conditions.

pH range:
6 - 10
Matrix:
Rigid, cross-linked agarose, spherical
Ligand:
Strep-Tactin XT
Storage temperature:
2...8 °C in 20% ethanol
Binding capacity:
~10 mg protein with Strep-tag II or Twin-Strep-tag/ml of resin
Ligand density:
~5 mg/ml resin
Flow rate:
300 cm/h