Unconjugated

Recombinant

HEK293 cells

Human

–20 °C

Less than 0,1 EU per μg by the LAL method.

Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7,5. Normally trehalose is added as protectant before lyophilisation. Measured by its binding ability in a functional ELISA. Immobilised Human B7-1, Fc Tag (Cat. No. ACRBB71-H5259) at 2 μg/ml (100 μl/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. ACRBCT4-H52A4) with a linear range of 0,1 - 2 ng/ml (QC tested). Measured by its binding ability in a functional ELISA. Immobilised Human B7-2, Fc Tag (Cat. No. ACRBCD6-H5257) at 2 μg/ml (100 μl/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. ACRBCT4-H52A4) with a linear range of 0,5 - 7,8 ng/ml (Routinely tested). Measured by its binding ability in a functional ELISA. Immobilised Ipilimumab at 5 μg/ml (100 μl/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. ACRBCT4-H52A4) with a linear range of 0,1 - 0,8 ng/ml (Routinely tested). Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. ACRBCT4-H52A4) on Protein A Biosensor, can bind Human B7-1, His Tag (Cat. No. ACRBB71-H5228) with an affinity constant of 0,52 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. ACRBCT4-H52A4) on Protein A Biosensor, can bind Human B7-2, His Tag (Cat. No. ACRBCD6-H5223) with an affinity constant of 1,6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

P16410-1

Y

Y

Y

CTLA4|CD152

CTLA-4

>95%

40,6 kDa

Low

Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7,5. Normally trehalose is added as protectant before lyophilisation.

Y

Powder,RT