Biosensis Alpha-Synuclein Rapid™ ELISA kit: human

The Biosensis Alpha-Synuclein Rapid™ ELISA kit is a sandwich ELISA that allows the quantification of human alpha-synuclein in less than 4 hours in human citrate-plasma, serum and CSF only if used as directed.

Complete ELISA kit with reagents and detailed protocols
Fast assay (<4 hours) without compromising accuracy and precision
Validated for human blood and CSF samples
Refer to the kit protocol for specific use instructions for blood and CSF application

This ELISA kit consists of a pre-coated sheep polyclonal anti-alpha-synuclein (aa: 116-131) capture antibody, a biotinylated mouse monoclonal anti-alpha-synuclein detection antibody (aa: 61-95) and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of alpha-synuclein present in samples and protein standards. A human alpha-synuclein positive control (QC sample) is provided to assure consistent assay performance.

Specificity: Human alpha-synuclein. Detection of individual alpha-synuclein isoforms has not yet been investigated. However, due to the nature of the assay antibodies, this ELISA kit is not expected to discriminate between various alpha-synuclein isoforms (monomeric, oligomeric, phosphorylated).

Cross-reactivity with beta- and gamma-synuclein was calculated based on increase or decrease of apparent endogenous α-synuclein concentrations when spiking beta- and gamma-synuclein into two human plasma samples. This ELISA shows very little cross-reactivity of 2,3% or less for beta- and gamma-synuclein.

: The ELISA kit box contains pre-coated strip plate, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.

: For research use only. Not for diagnostic or clinical purposes.

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