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ROS-ID™ total ROS/superoxide detection kit
ROS-ID™ total ROS/superoxide detection kit
  ENZOENZ51010
 :  
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ROS-ID™ total ROS/superoxide detection kit
  ENZOENZ51010
 :  

 

  • Pk:
    1 KIT
  • Description:
    ROS-ID® Total ROS/Superoxide detection kit
  • Seq:
    02
  • Storage conditions:
    −80 °C
  • Application:
    Flow cytometry, fluorescence microscopy, fluorescent detection and HTS
  • Size:
    1 kit

 

 

The kit is designed to directly monitor real time reactive oxygen species (ROS) production and Specifically Superoxide in live cells using fluorescence microscopy or flow cytometry.

  • Distinguishes between different reactive species, such as hydrogen peroxide, peroxynitrite and hydroxyl radicals
  • High sensitivity, specificity and accuracy for live cell studies
  • Compatible with major components of tissue culture media (phenol red, FBS and BSA)
  • Complete set of reagents, including ROS inducers and scavengers
  • Validated on microscopy, flow cytometry, and microplates

Through the combination of these two specific fluorescent probes, the kit provides a simple and specific assay for the real-time measurement of global levels of reactive oxygen species (ROS), and specifically superoxide in living cells. The Total ROS/Superoxide Detection Kit contains sufficient reagents for at least 200 microscopy assays or 50 flow cytometry assays using live cells (adherent or in suspension) or 2 x 96 microplate assays.

 : Kit contains Oxidative Stress Detection Reagent (Green), 300 nmoles, Superoxide Detection Reagent (Orange), 300 nmoles, ROS Inducer (Pyocyanin), 1 µmole, ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg and Wash Buffer Salts, 1 pack.