Order Entry
France
ContactUsLinkComponent
 
Human recombinant RANK ligand (from E. coli)
  PRSI92-558
 :  
Human recombinant RANK ligand (from E. coli)
  PRSI92-558
 :  

 

  • Pk:
    50 µG
  • Protein/peptide type:
    Recombinant
  • Source:
    E. coli
  • Species:
    Human
  • Tag sequence:
    N-6 His tag
  • Storage conditions:
    Lyophilized protein should be stored at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.
  • Protein synonyms:
    tumor necrosis factor ligand superfamily member 11|OPGL|TNFSF11|ODF|TRANCE|Receptor activator of nuclear factor kappa-B ligand|CD254|Osteoclast differentiation factor|RANK L
  • Protein/peptide name:
    RANK Ligand
  • Purity:
    > 95% as determined by reducing SDS-PAGE
  • Molecular weight:
    22.4 kD
  • Sequence:
    Ile140-Asp317
  • Formulation:
    Lyophilized from a 0.2 um filtered solution of 20mM Tris,150mM NaCl,pH8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

 

CD254, also known as RANKL, TNFSF11, TRANCE, OPGL and ODF, is a type II membrane protein of the tumour necrosis factor (TNF) superfamily, and affects the immune system and control bone regeneration and remodelling. RANKL is the ligand of nuclear factor (NF)-κB (RANK). When RANKL binds to RANK, it will undergo trimerisation and then bind to an adaptor molecule TNF receptor-associated factor 6 (TRAF6). This results in the activation of several downstream signallling cascades, including the NFκB, mitogen-activated protein kinases (MAPK), activating protein 1 (AP-1), and nuclear factor of activated T cells (NFATc1), resulting in the formation of multinucleated bone-resorbing osteoclasts. RANKL is widely expressed in skeletal muscle, thymus, liver, colon, small intestine, adrenal gland, osteoblast, mammary gland epithelial cells, prostate and pancreas.

Fusion-Tag: N-6 His tag

This recombinant protein can be used for biological assays. For research use only.