The CellTiter-Glo 2, 0 assay provides a homogeneous method of determining the number of viable cells in culture by quantitating the amount of ATP present, which indicates the presence of metabolically active cells.

• A luminescent cell viability assay for fast, easy, everyday use
• Reduced preparation time
• Convenient repeat use over multiple days
• Same great performance as classic CellTiter-Glo(R) assay

The CellTiter-Glo 2,0 assay provides a homogeneous method for determining the number of viable cells in culture by measuring the amount of ATP present, which indicates the presence of metabolically active cells. The CellTiter-Glo 2,0 assay is based on the original CellTiter-Glo assay chemistry but with improved storage convenience for easy implementation. The CellTiter-Glo 2,0 assay is provided as a single, ready-to-use reagent that can be stored at 4 °C for up to 2 months with >85% activity remaining or at room temperature for 1 week with >85% activity remaining. The CellTiter-Glo 2,0 assay is designed for use with multiwell plate formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo 2,0 Reagent) directly to cells cultured in serum-supplemented medium.

Cell washing, removal of medium and multiple pipetting steps are not required. The system detects as few as 15 cells/well in a 384-well format in 10 minutes after adding reagent. The homogeneous 'add-mix-measure' format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo 2.0 Assay generates a 'glow-type' luminescent signal, which has a half-life generally greater than three hours, depending on cell type and medium used. The extended half-life eliminates the need to use reagent injectors and provides flexibility for continuous or batch-mode processing of multiple plates.