Maxima™ H minus cDNA synthesis master mix, with dsDNase

Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

One-tube master mix for reduced pipetting steps and enhanced consistency in RT-qPCR results
Increased RT efficiency across a wide range of input RNA amounts and gene targets
High thermostability to allow RT reactions at 50 to 65°C temperature range
Integrated step of genomic DNA removal from RNA samples

The master mix contains Maxima™ H Minus Reverse Transcriptase (RT) and RiboLock™ RNase Inhibitor. Maxima™ H Minus RT is an advanced enzyme derived from M-MuLV RT by in vitro evolution. The enzyme features high thermostability, robustness, processivity, increased cDNA synthesis rate, and lack of RNase H activity. The recombinant RiboLock™ RNase Inhibitor effectively protects RNA template from degradation by RNases A, B, and C at temperatures up to 55 °C. The master mix also contains reaction buffer, dNTPs, oligo (dT)18, and random hexamer primers. A separate tube of No-RT control mix is provided for convenient RT negative control.

: Kit contains Maxima™ H Minus cDNA synthesis master mix, Maxima™ H Minus cDNA synthesis master mix No RT control, dsDNase, 10X dsDNase buffer, and nuclease-free water.

Restricted Products: To process your orders without delay, please provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:

• State issued document with your organization's Federal Tax ID Number
• State issued document with your organization's Resale Tax ID Number
• City or County issued Business License
• State Department of Health Services License
• Any other ID issued by the State that includes the business name & address

* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.