About this item
This antibody recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Type: Primary
Antigen: FBXO5
Clonality: Monoclonal
Clone: EMI1/1176
Conjugation: Purified
Epitope:
Host: Mouse
Isotype: IgG2a kappa
Reactivity: Human
Specifications
- Catalog No:
- BNUB1176-500
- BNUB1176-100
- Antigen symbol:
- FBXO5
- FBXO5
- Antigen name:
- EMI1
- EMI1
- Antigen synonyms:
- Early Mitotic Inhibitor-1 (EMI1)|Fbxo31|FBX5
- Early Mitotic Inhibitor-1 (EMI1)|Fbxo31|FBX5
- Antibody type:
- Primary
- Primary
- Clonality:
- Monoclonal
- Monoclonal
- Conjugation:
- Unconjugated
- Unconjugated
- Clone:
- Clone EMI1/1176
- Clone EMI1/1176
- Reactivity:
- Human
- Human
- Host:
- Mouse
- Mouse
- Gene ID:
- 37045
- 37045
- Isotype:
- IgG2a kappa
- IgG2a kappa
- Western blot:
- Yes
- Yes
- ImmunoChemistry:
- Yes
- Yes
- ImmunoFluorescence:
- Yes
- Yes
- Concentration:
- 0,2 mg/ml
- 0,2 mg/ml
- Storage buffer:
- PBS/0,05% BSA/0,05% azide
- PBS/0,05% BSA/0,05% azide
Specifications
Supplier: VWR Chemicals
Supplier: HEATHROW
Supplier: VWR Collection
Supplier: VWR Chemicals
Supplier: VWR Collection
Supplier: Merck
Supplier: VWR Chemicals
Supplier: VWR Chemicals
Supplier: VWR Collection
Supplier: VWR Collection
Supplier: KIMBERLY CLARK
Supplier: VWR Collection