Primary normal human dermal fibroblasts (NHDF) are isolated form the dermis of juvenile foreskin or adult skin from different locations like the face, the breasts, the abdomen, and the thighs.

NHDF can be used for wound healing studies and dermatological research to investigate diseases like scleroderma, fibrosarcoma, fibrosis, xeroderma pigmentosum, and histiocytoma. They are also important for cancer research, tissue regeneration, and tissue engineering studies.