Primary human skeletal muscle cells (SkMC) are isolated from different skeletal muscles (eg. musculus pectoralis major) from adult single donors. They are positive for sarcomeric myosin and negative for smooth muscle specific α-actin.

Primary human skeletal muscle cells (SkMC) are optimal for in vitro muscle studies. They proliferate very well in the mitogen-rich skeletal muscle cell growth medium. Fusion to myotubes with typical multinucleated syncytia can be induced by using the skeletal muscle cell differentiation medium.