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Anti-IgG Chicken Polyclonal Antibody (TRITC (Tetramethylrhodamine Isothiocyanate))
Anti-IgG Chicken Polyclonal Antibody (TRITC (Tetramethylrhodamine Isothiocyanate))
Catalog # IMMRIR1026
Supplier:  ImmunoReagents
Anti-IgG Chicken Polyclonal Antibody (TRITC (Tetramethylrhodamine Isothiocyanate))
Catalog # IMMRIR1026
Supplier:  ImmunoReagents

Specifications

  • Antibody type:
    Secondary
  • Antigen name:
    Immunoglobulin G
  • Antigen symbol:
    IgG
  • Clonality:
    Polyclonal
  • Conjugation:
    TRITC (Tetramethylrhodamine Isothiocyanate)
  • ELISA:
    Yes
  • Flow cytometry:
    Yes
  • Host:
    Chicken
  • ImmunoChemistry:
    Yes
  • ImmunoFluorescence:
    Yes
  • ImmunoPrecipitation:
    Yes
  • Isotype:
    IgG
  • Reactivity:
    Goat
  • Western blot:
    Yes
  • Environmentally Preferable:
  • Cross adsorption:
    No
  • Format:
    Purified IgG
  • Form:
    Lyophilised
  • Storage buffer:
    10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free
  • Storage temperature:
    2...8 °C
  • Concentration:
    1,50 mg/ml (E 1% at 280 nm = 13,0)
  • Shipping temperature:
    2...8 °C
  • Immunogen:
    Purified Goat IgG, whole molecule
  • Tested applications:
    Immunomicroscopy
  • Purification:
    Affinity purified using solid phase Goat IgG
  • Size:
    1 mg
  • Pk:
    1 mg

Specifications

About this item

TRITC (Tetramethylrhodamine isothiocyanate) is a popular rhodamine derivative pink in color but emits a red-orange color upon Emission at 580 nm. TRITC is frequently paired with FITC in double labeling experiments and conjugated antibodies are often used in immunofluorescence microscopy, flow cytometry, FLISA (fluorescent ELISA) and high throughput screening assays. These conjugates are best excited with the 532 nm spectral line of the He-Ne laser and are also recommended as a second color detection reagent in in situ hybridisation applications. (Excitation/Emission = 555 nm / 580 nm Emission Color = Orange to Red (Similar Dyes: Alexa Fluor 555, Cy3, DyLight 550)

  • Origin: US

ImmunoReagents' antibodies are suitable for most immunoassay formats and purified, cross adsorbed and conjugated based on the final product specfications.

Products will be accompanied with a standard product CoA to include application, purity and cross reactivity profiles.

Certifications: (EC) 142/2011 approved by APHIS, Reference Number is NC-TEC-0002
Ordering information: Products can by customised based on specific assay requirements: contact your VWR representative for more information.

Type: Secondary
Antigen: IgG
Clonality: Polyclonal
Clone:
Conjugation: TRITC (Tetramethylrhodamine Isothiocyanate)
Epitope:
Host: Chicken
Isotype: IgG
Reactivity: Goat