Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

• For affinity chromatography
• NHS pre-activated medium for coupling of small amino-containing proteins and peptides in process-scale applications
• NHS coupling method results in chemically stable ligand attachment
• BioProcess medium supported for industrial applications
• High level of pre-activation gives a high degree of substitution of the selected ligand
• Stable in commonly used aqueous buffers provided that the ligand withstand the pH or the chemical environment

NHS-activated Sepharose 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, NHS-activated Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support. This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.

NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.