Supplier: G-Biosciences

The staining of gels with silver stains often results in over-staining or unevenly stained gels. Silver D-Stain™ eliminates the need to repeat the electrophoresis.

Supplier: Thermo Fisher Scientific

Although optimising assay conditions is the best way to achieve optimum results, re-performing the gel electrophoresis process to test each new primary antibody or antibody concentration is time-consuming and expensive. Restore™ Western blot stripping buffer offers an effective alternative.

Supplier: G-Biosciences

Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Supplier: Cytiva

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Supplier: SIGMA ALDRICH MICROSCOPY

It contains p-Phenylenediamine diHCL (1 part) and catechol (2 parts). Commonly referred to as Hanker Yates reagent. Kit component in the Sigma Leukocyte peroxidase (myeloperoxidase) Procedure, 390A.

Supplier: VWR Chemicals

Destaining bags are designed to safely remove ethidium bromide from solution during overnight treatment. Each bag extracts up to 5 mg of ethidium bromide from solution, thus minimising the exposure of research personnel to this toxic material. The special absorbent mixture retains dye molecules in a convenient bag for removal and incineration.

Supplier: Merck

Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels.

Supplier: VWR Chemicals

VWR®, Fixing solution for isoelectric focusing in polyacrylamide ge

Supplier: VWR Chemicals

VisiGlo™ and Visiglo Plus™ HRP chemiluminescent substrates are luminol-based chemiluminescent substrates designed for rapid and sensitive detection of peroxidase-labelled conjugates. Sensitivity ranges from picogram levels, captured by VisiGlo™ HRP chemiluminescent substrate, down to femtogram levels, exhibited by VisiGlo™ Prime and VisiGlo™ Select. VisiGlo™ Select displays the highest sensitivity, ideal for detecting low abundance proteins. The signal generated by substrate incubation is sustained between 8 to 10 hours allowing you to perform multiple exposures over an extended period of time.

Supplier: Molecular Devices

The ScanLater™ Western Blot Assay Kits are a time-resolved fluorescence (TRF)-based assay, optimal for quantitating as little as femtogram protein samples.

Supplier: G-Biosciences

Ready to screen human cancer cell line blots.

Supplier: ENZO LIFE SCIENCES

Chemiluminescent acridinium esters are commonly used to label antibodies, proteins, nucleic acids, and some peptides, and are frequently used as detection reagents in immunoassays.

Supplier: G-Biosciences

These are ready to screen, normal tissue, eye tissue region blots from rat and rabbit. The eye tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult rat and rabbit eyes.

Supplier: Merck

Immobilon® Block Noise cancelling reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimised to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection.

Supplier: Rockland Immunochemicals

These accurate and sensitive kits are ideal for Western blotting or dot blotting methods. They offer flexibility with two unique substrates: Bright Blue TMB substrate and Stable Brown DAB substrate. Components are colour-coded for error-free results. With MaxTag™ you can run up to 40 (10×10cm) immunoblots.

Supplier: G-Biosciences

These are ready to screen, normal tissue, kidney tissue region blots from mouse and rat. The kidney tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse and rat kidney.

Supplier: G-Biosciences

The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

Supplier: ENZO LIFE SCIENCES

Whole-cell extract of human HL-60 leukemia cells.

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

Supplier: Merck

Luminata™ Western HRP substrates are a family of premixed, ready to use chemiluminescent reagents for the detection of HRP-based Westerns.

Supplier: ENZO LIFE SCIENCES

The CYTAG™ CGH labeling kit produces high quality data for comparative genomic hybridisation (aCGH) using as little as 0,25 µg of genomic DNA, without a need for pre-amplification.

Supplier: G-Biosciences

Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.

Supplier: ENZO LIFE SCIENCES

HIGHDEF® red IHC chromogen can be used in conjunction with peroxidase-based immunostaining systems.

Supplier: Merck

Immobilon® Western HRP substrates are compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.

Supplier: G-Biosciences

Single tissue and multiple species blot contains four protein lanes with an identical GenLysate™ tissue lysate from human, mouse and rat species, and a protein marker lane.

Supplier: G-Biosciences

The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

Supplier: Merck

Chemiluminescent HRP detection reagent for use on immunoblots, Western blotting, dot/slot blotting.

Supplier: G-Biosciences

Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.