You searched for: Nucleic Acid Reagents

Supplier: TriLink BioTechnologies

TriLink® N1-Methoxymethylpseudouridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

A modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-ethylpseudouridine can reduce the immunogenicity of the resulting mRNA.

Supplier: TriLink BioTechnologies

2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dUTP is incorporated in both DNA and RNA constructs to improve in vivo stability.

Supplier: TriLink BioTechnologies

CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.

Supplier: TriLink BioTechnologies

TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

Supplier: AGILENT

These QuikChange II kits provide improved fidelity, while maintaining greater than 80% mutation efficiency for single site mutagenesis.

Supplier: TriLink BioTechnologies

CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.

Supplier: TriLink BioTechnologies

CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.

Supplier: ENZO LIFE SCIENCES

Cyanine 3-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .

Supplier: G-Biosciences

T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes. The protein forms a right-handed nucleoprotein complex on single ssDNA called the presynaptic filament that can search for homology in duplex DNA and pair the recombining DNA molecules to form a DNA joint molecule. This process is aided by ssDNA binding proteins and recombination mediators. The filament is then elongated by DNA polymerase with the newly synthesised strand displacing the old strand. The newly synthesised duplex DNA can then act as a template for the next cycle to achieve exponential amplification of dsDNA. E.coli cells expressing recombinant T4 UvsX gene.

Supplier: ENZO LIFE SCIENCES

Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.

Supplier: ENZO LIFE SCIENCES

Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity.

Supplier: AGILENT

The fastest and latest generation of the market-leading QuikChange kits speeds up the protocol for performing single and multiple site-directed mutagenesis to less than 3 hours.

Supplier: Corning

The AxyPrep™ FragmentSelect Kits utilise a paramagnetic bead technology for quick high throughput optimised DNA size selection suitable for various next generation sequencing platforms. The AxyPrep™ FragmentSelect-I kit is optimised for Illumina next generation sequencing platforms and Life Technologies (SOLiD™) fragment size selection needs simplifying the library construction workflow.

Supplier: Thermo Fisher Scientific

GGCCGC sites and cuts best at 37 °C in O buffer.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Thermo Fisher Scientific

AATTC sites and cuts best at 37 °C in its own unique buffer. Exhibit 100% activity in the recommended buffer and reaction conditions.

Supplier: AGILENT

Dpn I is an ideal enzyme for cloning and southern blot analysis.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Thermo Fisher Scientific

FastDigest™ XhoI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest™ and FastDigest™ Green reaction buffers.

Supplier: ENZO LIFE SCIENCES

The Adenovirus BioProbe® labeled probe is prepared by nick translation of cloned fragments of the adenovirus 5 genome. The fragments constitute a total of 20 kb of DNA, approximately 56% of the genome. The probe is specific for adenovirus and, in addition to hybridization to adenovirus 5 sequences, the probe will cross hybridize with adenovirus types 4, 7, 11, 20, 40 and 41 due to their considerable sequence homology. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

Supplier: ENZO LIFE SCIENCES

The procedure for labeling of DNA probes with a polynucleotide 'tail' containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions. Terminal labeling is an ideal procedure for the labeling of oligonucleotides.

Supplier: ENZO LIFE SCIENCES

The Cytomegalovirus (CMV) BioProbe® labeled probe is prepared by nick translation of cloned fragments of the CMV Towne strain. The fragments include a total of 30-31 kb of DNA, approximately 20% of the CMV genome. The probe is specific for cytomegalovirus DNA. It does not hybridize to DNAs of other herpesviruses (i.e., herpes simplex virus or Epstein-Barr virus) but it has been found to hybridize to the DNA of CMV clinical isolates. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

Supplier: Thermo Fisher Scientific

NGGCC sites and cuts best at 50 °C in G buffer.

Supplier: ENZO LIFE SCIENCES

AMPIGENE® cDNA Synthesis Kit for efficient and sensitive cDNA synthesis, for downstream qPCR.