You searched for: Nucleic Acid Reagents
Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.
TriLink® N7-Methyl-3'-O-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine
Supplier: TriLink BioTechnologies
TriLink® N7-Methyl-3'-O-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine
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TriLink® 7-Deaza-2'-deoxyguanosine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This deoxyguanosine analog lacks the N7 nitrogen found in the natural guanine nucleobase. Replacement of this nitrogen atom with a carbon atom results in diminished structure of the major groove in DNA and may disrupt base stacking, but does not interfere with Watson-Crick base pairing. This is desirable for applications such as amplification and sequencing of GC-rich nucleic acid sequences.
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E.Z.N.A.® miRNA isolation kit
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® miRNA isolation kit uses a rapid procedure to isolate small RNAs, such as micro RNA (miRNA), small interfering RNA (siRNA), and small nuclear RNA (snRNA), from wide range of tissues and cells. The fast and efficient silica membrane based method isolates total RNA ranging in size from kilo based down to decamers. The kit also provides reagents and a procedure to enrich the population of RNAs that are 200 bases and smaller, which enhances the sensitivity of small RNA detection by solution hybridisation, Northern analysis, and other methods.
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TriLink® N1-Methyl-Pseudouridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
N1-Methyl-Pseudouridine-5'-Triphosphate is widely used in mRNA synthesis for therapeutic and vaccine development to improve mRNA functionality. This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.
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Cyanine 5-UTP (enhanced)
Supplier: ENZO LIFE SCIENCES
Cyanine 5-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP.
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Extracta Plus DNA kits
Supplier: Quantabio
Rapid extraction and purification of high-quality total DNA from cultured cells or tissue.
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Mag-Bind® Environmental DNA 96 Kit
Supplier: OMEGA BIO-TEK
Isolate DNA from soil and water samples using magnetic beads.
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TriLink® 2',3'-Dideoxyguanosine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
2',3'-Dideoxyguanosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddGTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.
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TriLink® 2',3'-Dideoxythymidine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
2',3'-Dideoxythymidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddTTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.
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Mag-Bind® Blood DNA HV Kit
Supplier: OMEGA BIO-TEK
Isolate DNA from up to 10 ml blood using magnetic beads.
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Fungal DNA kits, E.Z.N.A.®
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® Fungal DNA kits allow for the rapid and reliable isolation of high quality total cellular DNA from a wide variety of fungal species without the need for organic extraction.
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TriLink® N6-Methyladenosine-5'-Triphosphate
Supplier: TriLink BioTechnologies
N6-methyl adenosine (N6-methyl ATP) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs (Meyer et al.). N6-methyl ATP can substitute for ATP in some biological systems, and is a potent agonist for P2Y-purinoceptors in the guinea pig, taenia coli (Burnstock et al.).
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Fluorescein-12-ddUTP dideoxynucleotide pack for 3’-oligonucleotide labeling
Supplier: ENZO LIFE SCIENCES
The procedure for labeling of DNA probes with a polynucleotide 'tail' containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions.
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Formalin-fixed, paraffin-embedded (FFPE) tissue RNA kits, E.Z.N.A®
Supplier: OMEGA BIO-TEK
Selected protease digestion releases microgram amounts of RNA from FFPE samples. The purified nucleic acids, although highly fragmented, are suitable for a variety of downstream genomic and gene expression analyses. E.Z.N.A® spin column-based kits are ideal for low throughput applications while Mag-Bind® magnetic beads-based kits are designed specifically for high throughput users with automation capability.
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Genomic DNA isolation
Supplier: AGILENT
The RecoverEase DNA isolation kit recovers high-molecular-weight (100 to 500 kb) genomic DNA that is fully hydrated and ready for multiple applications the next day. DNA can be isolated from whole tissues such as liver, spleen, kidney, brain and testes without hazardous and tedious organic extractions or ethanol precipitations. The RecoverEase DNA isolation kit quickly isolates high-molecular-weight genomic DNA from a variety of tissues without organic solvent extractions or ethanol precipitation.
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E.Z.N.A.® Soil DNA kit
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® soil DNA kit is formulated to isolate high purity cellular DNA from soil samples typically containing humic acid and inhibitors of PCR. This kit has been successfully used to isolate DNA from tough-to-lyse bacteria, fungi, and algae that inhabit a range of samples including clay, sandy, peaty, chalky, and loamy soil samples. Isolated DNA can be used for most downstream applications including PCR, southern blotting, and SNP analysis.
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RNA isolation, viral RNA kit, E.Z.N.A.®
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® Viral RNA Kit is designed for the isolation of viral RNA from cell free fluids such as plasma, serum, urine and cell culture supernatant. The procedure completely removes contaminants and enzyme inhibitors making viral RNA isolation fast, convenient and reliable. The kit is also suitable for the isolation of total RNA from cultured cells, tissues and bacteria. RNA purified using the E.Z.N.A.® Viral RNA method is ready for all downstream applications such as RT-PCR.
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Blood and tissue DNA kit, CE IVD, Mag-Bind®
Supplier: OMEGA BIO-TEK
Mag-Bind® Blood and Tissue DNA Kit CE IVD offers a versatile method for the isolation of high-quality DNA from a wide variety of samples including fresh or frozen animal cultured cells and tissues, up to 250 µl whole blood, buccal swabs, up to 500 µl saliva, and dried blood spots.
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E.Z.N.A.® Plasmid Mini kit II, Q-spin (without lid)
Supplier: OMEGA BIO-TEK
This second generation plasmid miniprep kit yields 40 to 70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 ml to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.
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SLK Human siRNA Oligo Duplex (Locus ID 9748)
Supplier: OriGene
This SLK Human siRNA Oligo Duplex mediates apoptosis and actin stress fiber dissolution.
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Mag-Bind® Endo-Free plasmid 96 kit
Supplier: OMEGA BIO-TEK
The Mag-Bind® Endo-Free plasmid 96 kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin-free plasmid DNA in a high throughput format. By adding a quick endotoxin removal step, the Mag-Bind® Endo-Free plasmid 96 kit provides a flexible and fast solution for isolating endotoxin free plasmid on a small economical scale. The flexible system can be easily automated, thereby making it an ideal option for high throughput transfection screening as well as for other standard molecular biology techniques including restriction enzyme digestion.
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RNA isolation, E.Z.N.A.® HP total RNA kit
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from a small amount of cultured eukaryotic cells or tissues. This kit allows single or simultaneous processing of multiple samples in less than 40 minutes. Normally, 1×10⁷ eukaryotic cells or 25 to 30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
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E.Z.N.A.® blood DNA maxi kit
Supplier: OMEGA BIO-TEK
The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 10 ml whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.
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TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)
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TriLink® N1-Propylpseudouridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® N1-Propylpseudouridine-5'-Triphosphate
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TriLink® Uridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.
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Deoxynucleotide mix
Supplier: AGILENT
The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR.
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dNTP set
Supplier: Biotium
Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mm.
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TriLink® 2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddCTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.
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miRNA reference kit
Supplier: AGILENT
The Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments.