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Protein Purification

Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

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Affinity chromatography resin, chelating nickel

Affinity chromatography resin, chelating nickel

Supplier: G-Biosciences

Nickel chelating resin is an immobilised metal affinity chromatography (IMAC) resin that was specifically designed to utilise Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.

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Magnetic Antibody Purification System, Abcam

Supplier: Abcam

Magnetic Antibody Purification System (ab270532) is a convenient method to purify antibodies at a small scale with high recoveries.

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TEV Protease Kit

Supplier: Biotium

TEV protease kit that may be used for cleaving proteins or peptides containing the TEV protease recognition sequence E-N-L-Y-F-Q↓(S/G/A/M/C/H).

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Diluent M Concentrate (10x)

Diluent M Concentrate (10x)

Supplier: Abcam

Diluent M Concentrate (10X) (ab138908) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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HRV14 3C (PreScission) Protease Agarose Beads, Abcam

Supplier: Abcam

HRV14 3C (PreScission) Protease Agarose Beads, ab286883, (previously known as #M1029) is prepared by covalent coupling active HRV14 3C Protease to activated 4% cross-linked agarose beads.

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Rat TCS Antibody Purification Kit, Abcam

Supplier: Abcam

Rat TCS Antibody Purification Kit (ab270562) is designed for the purification of rat IgG fractions from tissue culture supernatants.

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Proteasome Purification Kit

Supplier: Enzo Life Sciences

Proteasome Purification Kit is a high integrity kit for the isolation and purification of proteasome complexes from biological samples such as cell lysates, tissue extracts, plasma, serum, confirmed by Western blot analysis.

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Rabbit affinity purification resin

Rabbit affinity purification resin

Supplier: ImmunoReagents

ImmunoReagents Rabbit IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Rabbit IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

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Sheep affinity purification resin

Sheep affinity purification resin

Supplier: ImmunoReagents

ImmunoReagents Sheep IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Sheep IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

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Sample Diluent NBS

Sample Diluent NBS

Supplier: Abcam

Sample Diluent NBS (ab221827) is part of the reagents, controls and accessories range. High-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates are available.

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Serum Antibody Purification Kit (Protein A), Abcam

Supplier: Abcam

Serum Antibody Purification Kit (Protein A) (ab109209) is prepared by coupling highly purified protein A to agarose beads and can therefore be used to purify IgG fractions from both serum and ascites fluid.

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IgG purification resin, Pearl™

Supplier: G-Biosciences

The Pearl™ IgG purification resin allows for the one-step purification of immunoglobulin G from serum. The resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

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HRP Stability Conjugate Reagent

HRP Stability Conjugate Reagent

Supplier: Abcam

Abcam's HRP Stability Conjugate Reagent (ab171537) was developed specifically for Horseradish Peroxidase (HRP) conjugates in solution.

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Sample Diluent NBP

Sample Diluent NBP

Supplier: Abcam

Sample Diluent NBP is part of the reagents, controls and accessories range.

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Antibody clean up kit, Pearl™

Antibody clean up kit, Pearl™

Supplier: G-Biosciences

The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.

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Affinity purification resins, anti-His, GenScript®

Supplier: Genscript

Anti-His affinity resins are designed for purification of His-tagged protein. Composed of agarose and conjugated THE™ anti-His monoclonal antibody, they are compatible with most commonly used reagents at certain concentrations.

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