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Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

Cellular senescence activity assay

Cellular senescence activity assay

Supplier: ENZO LIFE SCIENCES

Highly reproducible SA-β-gal activity kit

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Calcineurin cellular activity assay kit

Calcineurin cellular activity assay kit

Supplier: ENZO LIFE SCIENCES

A complete colorimetric assay kit for measuring cellular calcineurin (PP2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts PLUS human recombinant calcineurin is included as a positive control.

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Cellular senescence live cell analysis assay

Supplier: ENZO LIFE SCIENCES

Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.

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FLUOR DE LYS® HDAC fluorometric cellular activity assay kit

Supplier: ENZO LIFE SCIENCES

The HDAC fluorometric cellular activity assay allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.

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Glucose Uptake Cell-Based Assay Kit

Glucose Uptake Cell-Based Assay Kit

Supplier: Cayman Chemical

A cell-based assay for studying modulators of cellular glucose uptake.

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APO-BRDU™ kit, TUNEL Assay

Supplier: ENZO LIFE SCIENCES

Cellular assay, APO-BRDU™ kit

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Caspase-3 cell assay kit

Supplier: ENZO LIFE SCIENCES

This kit is a complete assay system for measuring caspase-3 and caspase-3-like activity (DEVDase) in cellular extracts.

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Cell counting kit-8

Cell counting kit-8

Supplier: ENZO LIFE SCIENCES

Cellular assay, Cell counting kit-8

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Cell-based assay calcein AM

Supplier: Cayman Chemical

Cellular assay, Cell-based assay calcein AM

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Glucose (HK) assay kit

Supplier: Merck

The Glucose (HK) Assay Kit is for the quantitative, enzymatic determination of glucose in food and other material.

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Glycolysis Cell-Based Assay Kit

Glycolysis Cell-Based Assay Kit

Supplier: Cayman Chemical

A colorimetric-based method for detecting L-lactate in culture medium.

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Phagocytosis assay kit (IgG FITC)

Supplier: Cayman Chemical

Phagocytosis Assay Kit (IgG FITC) for the identification of factors regulating the phagocytic process in vitro.

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Calcium assay kit

Supplier: Cayman Chemical

For the determination of total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates.

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APO-BRDU™ kit, TUNEL Assay

APO-BRDU™ kit, TUNEL Assay

Supplier: Tonbo Biosciences

The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.

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Adipogenesis assay kit

Supplier: ENZO LIFE SCIENCES

Convenient method for the enhanced induction and detection of adipogenesis, in the classic 3T3L-1 model.

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Calcium assay kit, FluoForte®

Calcium assay kit, FluoForte®

Supplier: ENZO LIFE SCIENCES

FluoForte® Calcium Assay Kits detect mobilisation of intracellular calcium utilising a fluorogenic calcium-binding dye optimised for superior cell-permeability and retention. The self-quenching dye undergoes an electronic change upon binding of calcium, resulting in a several order of magnitude greater fluorescence.

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Triglyceride colorimetric assay kit

Supplier: Cayman Chemical

Triglyceride Colorimetric Assay Kit is a simple, reproducible and sensitive tool for assaying triglycerides in plasma, serum, cell lysates and tissue homogenate samples.

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Cytoplasmic membrane labelling kits, CellBrite™

Cytoplasmic membrane labelling kits, CellBrite™

Supplier: Biotium

CellBrite kits are designed to label cell cytoplasmic membranes with a fluorescent membrane dye pre-dissolved as an optimised staining solution. By combining CellBrite membrane stains, it is possible to label multiple cell populations with distinctive colours, a capability useful for the studies of cell-cell interactions.

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Millicell® µ-Migration Assay Kit

Millicell® µ-Migration Assay Kit

Supplier: Merck

This Kit overcomes the limitations of traditional multiwell migration assays. Its microfluidic, low-volume technology promotes a stable, diffusion-generated concentration gradient that is consistently linear and lasts for more than 48 hours.

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ViaFluor® 488 live cell microtubule stain 1000X in DMSO

Supplier: Biotium

Stain microtubules in live cells with green fluorescence.

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Ubiquitinylation kit

Ubiquitinylation kit

Supplier: ENZO LIFE SCIENCES

Versatile tool for generation of ubiquitin-E2 thioesters.

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Reporter gene assays, SpectraMax® DuoLuc™ reporter assay kit

Reporter gene assays, SpectraMax® DuoLuc™ reporter assay kit

Supplier: Molecular Devices

SpectraMax® DuoLuc™ reporter assay kit offers sensitive detection of dual luciferase reporter gene assays, which are important tools for monitoring gene expression and other cellular events.

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FluoForte™ Reagent

Supplier: ENZO LIFE SCIENCES

Calcium dye

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Fluorescent nerve terminal probes and stains

Fluorescent nerve terminal probes and stains

Supplier: Biotium

Nerve terminal probes are a series of fluorescent cationic styryl dyes developed to follow synaptic activity. These dyes have a lipophilic tail at one end and a hydrophilic cationically charged head group at the other end. The dyes vary in the number of carbons in the lipophilic tail (m) and the number of double bonds linking the two aromatic rings in the dye (n). AM dyes are similar to FM dyes, but contain an additional formaldehyde-fixable amino group in the cationic head.

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Reagents for Color-de-Lys™ HDAC colorimetric activity assay kit

Supplier: ENZO LIFE SCIENCES

Cellular assay, Color-de-Lys™ deacetylated standard, 30 µl

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Thiol Detection Assay Kits

Thiol Detection Assay Kits

Supplier: Cayman Chemical

A simple, reproducible, and sensitive tool for assaying free thiol content in samples.

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Red hydrogen peroxide assay kit

Supplier: ENZO LIFE SCIENCES

Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.

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ApoSENSOR™ Cell viability assay kit

Supplier: ENZO LIFE SCIENCES

The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).

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sPLA2 activity kit

Supplier: ENZO LIFE SCIENCES

For the quantitative determination of sPLA2 in culture supernatants, cell lysates, and buffers from any species.

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Autophagy detection tests, CYTO-ID®

Supplier: ENZO LIFE SCIENCES

CYTO-ID can monitor autophagy in live cells and autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles.

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