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Buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++

Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++

Supplier: Stemcell Technologies

Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.

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Hanks' Balanced Salt Solution with 10 mM HEPES, without Phenol Red

Hanks' Balanced Salt Solution with 10 mM HEPES, without Phenol Red

Supplier: Stemcell Technologies

Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Molekula

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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ALDEFLUOR™ Assay Buffer

ALDEFLUOR™ Assay Buffer

Supplier: Stemcell Technologies

Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.

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Fixation Buffer

Fixation Buffer

Supplier: Stemcell Technologies

Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.

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HEPES Buffer Solution (1 M)

HEPES Buffer Solution (1 M)

Supplier: Stemcell Technologies

HEPES buffer solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture and degree of chemical definition required for the cell culture application.

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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer) pH 8,0

Supplier: Molekula

Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer) pH 8,0

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: TREVIGEN

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, PBS solution 10X concentrate (phosphate buffered saline)

Supplier: TREVIGEN

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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EasySep™ Red Blood Cell Lysis Buffer

EasySep™ Red Blood Cell Lysis Buffer

Supplier: Stemcell Technologies

EasySep™ Red blood cell lysis buffer is for use as directed with EasySep™ or RoboSep™ whole blood kits for the lysis of red blood cells prior to separation from whole blood.

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RoboSep™-C PBS/EDTA Buffer

RoboSep™-C PBS/EDTA Buffer

Supplier: Stemcell Technologies

PBS buffer containing EDTA.

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Dulbecco's phosphate-buffered saline (DPBS) without Ca²⁺ and Mg²⁺

Dulbecco's phosphate-buffered saline (DPBS) without Ca²⁺ and Mg²⁺

Supplier: PROMOCELL

These dulbecco's PBS solution are used in tissue culture, cell isolation procedures and molecular biology.

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Dulbecco's Phosphate Buffered Saline (DPBS), with 2% Fetal Bovine Serum

Dulbecco's Phosphate Buffered Saline (DPBS), with 2% Fetal Bovine Serum

Supplier: Stemcell Technologies

Dulbecco's Phosphate Buffered Saline with 2% fetal bovine serum is recommended for use as a buffer to dilute or wash nucleated cells.

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