Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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VWR®, Rapid Transfer buffer 10X

Supplier: VWR Chemicals

Rapid transfer buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively.

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VWR® Phosphate Buffered Saline (PBS) with 0,05% Tween®20

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) with 0,05% Tween® 20, pH 7,5 is a ready-to-use 1× concentration.

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VWR® AVS TITRINORM, Cesium chloride/lanthanum chloride buffer solution acc. to schinkel for atomic absorption spectroscopy (aas)

Supplier: VWR Chemicals

This material is intended for use as an ionisation buffer for AAS

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VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR® AVS TITRINORM Buffer Solution, pH 9 (at 20 °C), Borate free

Supplier: VWR Chemicals

Solution prepared from AnalaR® NORMAPUR® grade analytical reagents.

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VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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VWR®, Phosphate Buffered Saline (PBS), pH 7,4

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate and potassium chloride. PBS helps to maintain a constant pH, is isotonic and non toxic to cells, and provide cells with water and essential inorganic ions. This formulation does not contain calcium and magnesium.

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VWR®, Tris-borate-EDTA (TBE) buffer

Supplier: VWR Chemicals

TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).

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VWR®, Buffers, Sodium acetate

Supplier: VWR Chemicals

Sodium acetate buffer D, VWR®

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J.T.Baker®, Sheath fluid, 3000/3500

Supplier: Avantor

Sheath fluid 3000/3500, J.T.Baker®

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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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VWR® AVS TITRINORM Buffer Solutions, CRMs, at 25 °C

Supplier: VWR Chemicals

pH Buffer solution, Carbonate, pH value: 10,01, Reference temperature: 25 °C, VWR®, Colourless

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VWR®, Cell culture salt solutions, Dulbecco's Phosphate Buffered Saline (DPBS)

Supplier: VWR Chemicals

DPBS is commonly used salt solution in cell enumeration as a diluent, for rinsing cells and as a buffer in many chromatographic procedures. DPBS is also used in FACS (Fluorescence-Activated Cell Sorting) process to wash and re-suspend cells during the dissociation process, as well as for many analytical methods or biochemical treatments.

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VWR® AVS TITRINORM Buffer Solutions, 20 °C

Supplier: VWR Chemicals

Solutions prepared from AnalaR® NORMAPUR® grade analytical reagents.

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VWR® AVS TITRINORM pH Buffer Solutions in Sachets, 20 °C

Supplier: VWR Chemicals

For calibration of pH meters.

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VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

Supplier: VWR Chemicals

TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.