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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Factor XA protease digestion buffer

Supplier: Thermo Fisher Scientific

Factor XA Protease Digestion Buffer

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Denaturing lysis buffer

Supplier: Thermo Fisher Scientific

Denaturing lysis buffer, Thermo Fisher Scientific

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TRIS-glycine native sample buffer (4X), pH 8,6

Supplier: Thermo Fisher Scientific

Loading Buffer, TRIS-Glycine Native Sample Buffer (4X)

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TRIS-glycine-SDS running buffer (10X), pH 8,3

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-glycine-SDS running buffer (10X)

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TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

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Extraction reagents

Supplier: Enzo Life Sciences

Concentrated lysis buffer for preparation of cell and tissue extracts.

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J.T.Baker® Cell Lysis Solution, Biotech Reagent

J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: Avantor

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

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Buffer, tris(hydroxymethyl)aminomethane hydrochloride, 1 M solution, pH 7,4, RNase free

Supplier: Thermo Fisher Scientific

Cloning Buffer, TRIS buffer solution pH 7,4 (1 mol/l)

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Blocking solution, BLOK™ Casein

Blocking solution, BLOK™ Casein

Supplier: G-Biosciences

BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.

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Protein extraction kit, yeast PE LB™

Protein extraction kit, yeast PE LB™

Supplier: G-Biosciences

Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.

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Laemmli SDS sample buffer, reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, reducing, 4X

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Buffers, CAPS, 0.5 M solutions

Supplier: Thermo Fisher Scientific

CAPS buffer solution, 0,5 M, pH value: 11,0, Liqui

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Tween 20 washing buffer, 0,5% in PBS (10X)

Supplier: Thermo Fisher Scientific

Washing buffer, Tween 20 washing buffer, 0,5% in PBS (10X)

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Transfer buffer, Tris-glycine,10X, AccuGENE™

Transfer buffer, Tris-glycine,10X, AccuGENE™

Supplier: LONZA

AccuGENE™ electrophoresis buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.

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Neutralising reagent

Supplier: Enzo Life Sciences

Neutralising buffer for low pH solutions and for use with cyclic nucleotide and isoprostane kits.

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TE buffer, pH 7,6, RNAse free

Supplier: Thermo Fisher Scientific

TE buffer is a commonly used buffer solution in procedures involving DNA or RNA. TE buffer functions to solubilise DNA or RNA while protecting it from degradation. This buffer is used at different pH values and has become the standard buffer for the storage of nucleic acids.

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TRIS-buffered saline (TBS, 10X), with 0,5% Tween 20

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X), with 0,5% Tween 20

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Tween-20 solution (10%)

Supplier: Enzo Life Sciences

This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.

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TRIS-buffered saline (TBS, 10X, high salt) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X, high salt)

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Lysis buffers, red blood cells

Lysis buffers, red blood cells

Supplier: G-Biosciences

These DNase- and RNase-free buffers and reagents are designed for high quality research.

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Buffer, HEPES solution (1 mol/L), ULTROL Grade, Millipore®

Supplier: Merck Millipore (Calbiochem‎)

HEPES buffer solution (1 mol/l)

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Triethylammonium acetate buffer, BioUltra, volatile buffer, Fluka®

Supplier: Honeywell Chemicals

Triethylammonium acetate buffer, 1,0 M in H₂O, pH value: 7,0

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RIPA Lysis and Extraction Buffer

Supplier: G-Biosciences

A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.

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Nitrocellulose stripping buffer (10X)

Supplier: Thermo Fisher Scientific

Electrophoresis, Nitrocellulose Stripping Buffer, 10X

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AccuGENE® 1M Tris HCl buffer

Supplier: LONZA

AccuGENE™ Molecular Biology Buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.

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10X TBE buffer, liquid, Corning®

10X TBE buffer, liquid, Corning®

Supplier: Corning

TBE (Tris-Borate-EDTA) has applications in DNA and RNA agarose gel electrophoresis and polyacrylamide gel electrophoresis.

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pH buffer solutions, colour coded

pH buffer solutions, colour coded

Supplier: Hach

For calibration of pH meters

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AMPD, 0.2 M Buffer Solutions

Supplier: Thermo Fisher Scientific

2-Amino-2-methyl-1,3-propanediol buffer, colorless and odorless.

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Buffers, acetate, 1M solutions, pH 3,0 to 5,5

Supplier: Thermo Fisher Scientific

Acetate, 1M buffer solution, pH, 5,0, Liquid

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HEPES, 1,0 M buffer solutions

Supplier: Thermo Fisher Scientific

HEPES buffer solution, 1 mol/L, pH value: 8,5

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